基于质谱的组蛋白修饰鉴定技术及新修饰发现  

Mass spectrometry-based technologies for histone PTM analysis and novel PTM discovery

作　　者：陈凯峰 赵雯思 章珺 赵帅[3] 李海涛[3] 谭敏佳 Kaifeng Chen;Wensi Zhao;Jun Zhang;Shuai Zhao;Haitao Li;Minjia Tan(State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Basic Medical Sciences,Tsinghua University,Beijing 100084,China)

机构地区：[1]中国科学院上海药物研究所,原创新药全国重点实验室,上海201203 [2]中国科学院大学,北京100049 [3]清华大学基础医学院,北京100084

出　　处：《中国科学:化学》2025年第4期977-985,共9页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金重大研究计划集成项目(编号:92153302)资助。

摘　　要：组蛋白修饰是表观遗传调控的重要机制,在生命活动调节中发挥基础作用.组蛋白修饰高度复杂,目前已报道30多种化学修饰类型和数百个修饰位点,因此组蛋白修饰全景高效表征技术的开发对于组蛋白修饰解析和后续生物学功能研究至关重要.近年来随着高分辨质谱技术的发展,质谱技术已成为系统性鉴定组蛋白修饰的主要手段.质谱通过检测修饰引入的质量位移,一方面可以对不同组蛋白修饰类型进行无偏向性鉴定和定量分析,另一方面还能检测到未知翻译后修饰的质量位移,成为新型翻译后修饰发现的最关键技术之一.本评述关注近年来基于质谱的组蛋白修饰鉴定技术研究,并且总结了近年来报道的新型组蛋白修饰.Histone post-translational modifications(PTMs)serve as a crucial mechanism in epigenetic regulation and play a fundamental role in the regulation of biological activities.The complexity of histone modifications is remarkable,with over 30 types of modifications and hundreds of modification sites reported,which makes the development of global histone PTM analysis techniques critical.In recent years,with advancements in mass spectrometry technology,proteomics has emerged as a powerful tool for histone modification identification.By detecting mass shifts induced by PTMs,mass spectrometry enables the unbiased identification of multiple histone modification types,as well as the detection of new PTMs with unknown amino acid mass shifts.In this review,we focus on recent developments in mass spectrometry-based techniques for global histone PTM identification and summarize recently discovered new types of histone modifications.

关 键 词：蛋白组学 组蛋白修饰 新型翻译后修饰 

分 类 号：Q51[生物学—生物化学]