杂交捕获测序技术应用与研究进展  

Application and research advances of the hybridization capture sequencing technology

作　　者：陈明林 骆佳[1] 匡卫民 于黎[1] CHEN MingLin;LUO Jia;KUANG WeiMin;YU Li(State Key Laboratory for Conservation and Utilization of Bio-Resource in Yunnan,School of Life Sciences of Yunnan University,Kunming 650500,China)

机构地区：[1]云南大学省部共建云南生物资源保护与利用国家重点实验室,生命科学学院,昆明650500

出　　处：《中国科学:生命科学》2025年第4期773-789,共17页Scientia Sinica(Vitae)

基　　金：国家重点研发计划(批准号:2022YFC2602500);国家自然科学基金(批准号:32260133);云南省基础研究项目(批准号:202301AT070234,202301AU070214);云南省教育厅科学研究基金(批准号:2024Y016);中央引导地方科技发展资金(批准号:202407AB110004);云南省中越濒危非人灵长类保护遗传学国际联合实验室(批准号:202403AP140011);云南省兴滇英才支持计划科学家工作室和云南省兴滇英才支持计划青年人才项目资助。

摘　　要：随着测序技术的不断发展,杂交捕获测序技术通过设计特定的生物素化探针与目标DNA序列进行杂交,对特定基因或基因组目标区域进行富集和测序,实现了利用较少的数据量就能对目标区域变异位点进行快速、准确地检测,大大降低了测序成本.本文系统综述了杂交捕获测序技术在动物、植物、微生物以及医学研究领域中的广泛应用和研究进展,揭示了杂交捕获测序技术在古DNA研究、非损伤性样本宿主DNA富集、疾病、病毒和病原体检测等研究领域的巨大潜力.此外,文章探讨了杂交捕获在应用和发展过程中遇到的问题与解决方法,并对杂交捕获技术的优化方向进行了展望,以期为该技术的进一步应用和发展提供参考.With the development of sequencing technology,the hybridization capture sequencing technology can enrich and sequence-specific genes or genomic target regions by designing specific biotinylated baits to hybridize with the target DNA sequences.This method achieves rapid and accurate detection of the variant loci of the target regions with a smaller amount of data and greatly reduces sequencing costs.In this paper,we systematically reviewed the extensive application and research advances of the hybridization capture sequencing technology in animal,plant,microorganism,and medical study areas.This method highlights the great potential in ancient DNA research,host DNA enrichment of non-invasive samples,the detection of diseases,viruses,and pathogens.In addition,the problems and solutions encountered in the application and development of the hybridization capture sequencing are discussed,and the optimization direction of this technology is prospected.This review provides a reference for further application and development of the hybridization capture sequencing technology.

关 键 词：杂交捕获测序 生物素化探针 高通量测序 DNA富集 DNA文库 

分 类 号：Q78[生物学—分子生物学]