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作 者:王杰青 查继兰 沈永宝 WANG Jieqing;ZHA Jilan;SHEN Yongbao(College of Forestry,Co-Innovation Center for Sustainable Forestry in SouthernChina,Nanjing Forestry University,Nanjing 210037,China;Gold Mantis School of Architecture,Soochow University,Suzhou Jiangsu 215125,China)
机构地区:[1]南京林业大学林学院/南方现代林业协同创新中心,南京210037 [2]苏州大学金螳螂建筑学院,江苏苏州215125
出 处:《种子》2025年第3期17-25,共9页Seed
基 金:江苏省林业科技创新与推广项目(LYKJ[2021]03)。
摘 要:南京椴种子是具有深休眠特性的木本种子,通过H_(2)SO_(4)+GA_(3)+低温层积处理可以迅速打破其休眠。为了探究南京椴种子萌发过程中胚乳变化和代谢情况,用蛋白质组学和生物信息学方法分析由H_(2)SO_(4)+GA_(3)+低温层积处理的种子。结果表明,质谱鉴定出16种蛋白质,出现显著变化,这主要与营养库的活性,程序性细胞死亡和氧化还原反应有关。在解除休眠之前,最重要的生物学过程是程序性细胞死亡;解除休眠后,最重要的生物学过程是细胞分解代谢。增加了4个不同的蛋白质聚集途径:糖酵解/糖异生、次生代谢产物合成、氨基酸生物合成乙醛酸和乙醛酸代谢。释放休眠后,最重要的生物学过程是应激反应,这增加了代谢和氨基酸合成的两个途径。差异蛋白相互作用网络分析结果表明,该网络包含25个节点和159个连接边。WGCNA方法用于构建蛋白质共表达网络。GO功能和途径注释结果表明,共表达网络中的蛋白质与光合作用、葡萄糖代谢和金属离子反应等有关。此外,参与植物激素合成,碳水化合物的生物合成等途径。Tilia miqueliana seeds exhibit deep dormancy characteristics typical of woody plant species,which can be effectively broken through combined H_(2)SO_(4)and GA_(3)treatment followed by cold stratification.To investigate endosperm alterations and metabolic dynamics during seed germination,proteomic and bioinformatics approaches were employed to analyze treated seeds subjected to H_(2)SO_(4)+GA_(3)+cold stratification.The results showed that mass spectrometry identified 16 significantly altered proteins primarily associated with storage mobilization activity,programmed cell death(PCD),and redox reactions.It was revealed distinct biological process was mainly PCD before dormancy releasing while cellular catabolism became prominent post-dormancy after dormancy releasing.Four protein aggregation pathways showed marked activation:glycolysis/gluconeogenesis,secondary metabolite biosynthesis,amino acid biosynthesis and glyoxylate metabolism.Following dormancy termination,stress response mechanisms dominated biological processes,accompanied by enhanced metabolic and amino acid synthesis pathways.Differential protein interaction network analysis constructed a network containing 25 nodes and 159 edges.Weighted Gene Co-expression Network Analysis(WGCNA)methodology established protein co-expression networks,with Gene Ontology(GO)functional and pathway annotation indicating their involvement in biological processes including photosynthesis,glucose metabolism,and metal ion responses.Furthermore,these networks participated in phytohormone biosynthesis and carbohydrate biogenesis pathways.
分 类 号:S792.36[农业科学—林木遗传育种]
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