基于UPLC-Q-TOF-MS技术的通脉养心丸入血、入心脏组织成分分析及其质谱裂解规律研究  

UPLC-Q-TOF-MS Reveals Components of Tongmai Yangxin(通脉养心丸)Pills in Blood and Heart and Their Fragmentation Patterns

作　　者：唐艳 陈婷[3] 刘思琦 陈婷 TANG Yan;CHEN Ting;LIU Siqi;CHEN Ting(Hunan Provincial Key Laboratory of Cardio-Brain Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208;Hunan Provincial Key Laboratory of Traditional Chinese Medicine Powder and Innovative Drug Research,Changsha 410208;College of Acupuncture&Moxibustion and Tuina,Hunan University of Chinese Medicine,Changsha 410208;State Key Laboratory of Component-based Chinese Medicine,Tianjin 301617)

机构地区：[1]湖南中医药大学,中西医结合心脑疾病防治湖南省重点实验室,长沙410208 [2]湖南省中药粉体与创新药物研究省部共建国家重点实验室培育基地,长沙410208 [3]湖南中医药大学,针灸推拿与康复学院,长沙410208 [4]省部共建组分中药国家重点实验室,天津301617

出　　处：《中药药理与临床》2025年第2期27-32,共6页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(编号:82405131);国家自然科学基金预研项目(编号:22023YYJJ04);中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(编号:23PTKF1019);组分中药国家重点实验室资助课题(编号:CBCM2024103);高层次人才科研启动基金(编号:1101/0001020);湖南中医药大学本科生科研创新基金项目(编号:2023BKS029)。

摘　　要：目的:本研究采用超高效液相色谱-四极杆-飞行时间串联质谱(UPLC-Q-TOF-MS)技术对通脉养心丸大鼠体内血清及心脏组织成分进行快速定性分析。方法:选择Waters ACQUITY UPLC BEH C_(18)色谱柱,采用正负离子模式下质谱扫描,根据样品采集模式、一级质谱加合离子信息、二级碰撞能量、二级碎片信息等多级质谱信息对化合物进行鉴定分析。结果:从给药通脉养心丸大鼠血清中共鉴定出17个化合物,包括9个黄酮类、4个皂苷类、3个五环三萜类、1个有机酸类化合物。从心脏组织样本中共鉴定出3个化合物,包括2个黄酮类和1个皂苷类。结论:本研究辨析了通脉养心丸入血及心脏组织原型及其代谢成分,揭示了通脉养心丸化学成分群在体内变化和传递过程,为通脉养心丸的质量控制、临床应用以及新药研发提供实验依据。Objective:In this study,ultra-high performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS)was employed to rapidly and qualitatively analyze the components of Tongmai Yangxin(通脉养心丸)pills in the serum and cardi-ac tissue samples of rats.Methods:The Waters ACQUITY UPLC BEH C18 column was used and MS scanning was carried out in positive and negative ion modes.The MS information such as data acquisition mode,adduct ion in MS1,collision energy in MS2,and fragment in MS2 was used to identify the compounds.Results:A total of 17 compounds were identified from the serum samples of rats treated with Tongmai Yangx-in pills,including 9 flavonoids,4 saponins,3 pentacyclic triterpenoids,and 1 organic acid.A total of 3 compounds were identified from the heart tissue samples,including 2 flavonoids and 1 saponin.Conclusion:This study analyzed the original and metabolized components of Tong-mai Yangxin pills in the blood and heart to reveal the changes and transmission processes of chemical components in vivo,providing an experi-mental basis for the quality control,clinical application,and new drug development of Tongmai Yangxin pills.

关 键 词：通脉养心丸 超高效液相色谱-四极杆-飞行时间质谱法 定性分析 体内化学成分 

分 类 号：R284.1[医药卫生—中药学]