鼻敏水HPLC指纹图谱、化学模式识别及含量测定研究  

Study on the HPLC fingerprint,chemical pattern recognition and content determination of Bimin Shui

作　　者：韦瑀龙 黄燕琼 谢广源 潘绍棉 韦旭日 蓝晓庆 WEI Yulong;HUANG Yanqiong;XIE Guangyuan;PAN Shaomian;WEI Xuri;LAN Xiaoqing(The First Affiliated Hospital of Guangxi University of Chinese Medicine,Guangxi Nanning 530023,China;Guangxi Xianzhu Pharmaceutical Co.Ltd.,Guangxi Liuzhou 545000,China)

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023 [2]广西仙茱制药有限公司,广西柳州545000

出　　处：《中药与临床》2025年第2期23-27,33,共6页Pharmacy and Clinics of Chinese Materia Medica

基　　金：广西中医药管理局自筹经费项目(GXZYA20220024);广西中医药优秀人才培养项目(桂中医药科教【2022】14号)。

摘　　要：目的:建立鼻敏水(BM)的高效液相指纹图谱和含量测定方法,并对指纹图谱数据进行化学模式识别分析。方法:采用高效液相色谱法测定10批BM样品,利用相似度评价软件建立其共有特征图谱,将共有峰与处方药味相关联,并指认部分共有峰;以各共有峰峰面积为原始数据,采用SPSS软件做聚类分析(HCA),SIMCA软件做主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA),以变量投影重要性分析值(VIP)大于1筛选质量差异物;建立HPLC法同时测定BM中绿原酸、丹皮酚、木兰花碱、芍药苷含量。结果:BM指纹图谱共标定16个共有峰,10批样品色谱图相似度在0.972~0.999之间,对各共有峰进行药材归属,并指认出峰4(木兰花碱)、峰6(绿原酸)、峰14(丹皮酚)。HCA和PCA结果均显示10批样品(B1~B10)可分为2大类,B3~B5、B7、B8为一类,其余为一类。OPLS-DA筛选得到BM的质量差异物共5个,VIP从大到小依次为峰14(丹皮酚)、峰6(绿原酸)、峰13、峰1、峰3。10批样品中绿原酸、丹皮酚、木兰花碱、芍药苷4种成分的含量分别为28.87~50.77,50.20~110.25,21.06~46.87,97.13~146.98μg·mL^(-1)。结论:所建立的HPLC指纹图谱和含量测定方法能较全面的反映产品质量信息,方法操作简便快捷、重复性好,可为BM的质量控制和评价提供参考。Objective:To establish HPLC fingerprint and content determination method of Bimin Shui(BM),then analyze the HPLC fingerprint by chemical pattern recognition.Methods:The HPLC method was used to determine the contents in 10 batches of BM samples,and the shared characteristic chromatogram was established using the Chromatographic Fingerprint Similarity Evaluation System.The shared peaks were associated with the formula ingredients,and some of the shared peaks were identified.The original data of the shared peaks'peak areas were used to perform cluster analysis(HCA),principal component analysis(PCA),and orthogonal partial least squares-discriminant analysis(OPLS-DA)using SPSS software,and the variable projection importance analysis value(VIP)greater than 1 was used to screen quality difference substances.An HPLC method was established to simultaneously determine the contents of chlorogenic acid,paeoniflorin,magnolol,and paeoniflorin in BM.Results:A total of 16 shared peaks were identified in the BM fingerprint chromatogram,and the similarity of the chromatograms of the 10 batches of samples was between 0.972 and 0.999.The herbs to which the shared peaks belonged were identified,and peak 4(magnolol),peak 6(chlorogenic acid),and peak 14(paeoniflorin)were identified.The HCA and PCA results showed that the 10 batches of samples(B1 to B10)could be clustered into two categories.B3,B4,B5,B7 and B8 were grouped into one category,and the other batches of products were grouped into one category.OPLSDA screened out a total of 5 quality difference substances in BM,with VIP in descending order being peak 14(paeoniflorin),peak 6(chlorogenic acid),peak 13,peak 1,and peak 3.The contents of chlorogenic acid,paeoniflorin,magnolol,and paeoniflorin in the 10 batches of samples were 28.87 to 50.77,50.20 to 110.25,21.06 to 46.87,and 97.13 to 146.98μg·mL^(-1),respectively.Conclusions:The HPLC fingerprint and content determination established in this study can relatively comprehensive reflect the product quality information and were simpl

关 键 词：鼻敏水 HPLC指纹图谱 化学模式识别 含量测定 质量控制 

分 类 号：R284.1[医药卫生—中药学]