糖类对美国白蛾生长发育及消化酶活性的影响  

Effect of carbohydrates feeding on development and digestive enzyme activity of Hyphantria cunea

作　　者：姜璠 邹航 徐震霆 葛昆章 温玄烨 王越 JIANG Fan;ZOU Hang;XU Zhenting;GE Kunzhang;WEN Xuanye;WANG Yue(Center for Biological Disaster Prevention and Control,National Forestry and Grassland Administration/Key Laboratory of National Forestry and Grassland Administration on Forest and Grassland Pest Monitoring and Warning,Shenyang,Liaoning 110034,China;Chinese Academy of Inspection and Quarantine,Beijing 100176,China;Honghuaerji Forestry Bureau,Hulunbuir,Inner Mongolia 021112,China)

机构地区：[1]国家林业和草原局生物灾害防控中心/林草有害生物监测预警国家林业和草原局重点实验室,辽宁沈阳110034 [2]中国检验检疫科学研究院,北京100176 [3]红花尔基林业局,内蒙古呼伦贝尔021112

出　　处：《福建农林大学学报(自然科学版)》2025年第3期317-324,共8页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家重点研发计划项目(2021YFD1400300);国家林业和草原局生物灾害防控中心优青项目(FKQX2022001)。

摘　　要：【目的】研究糖类对美国白蛾生长发育及消化酶活性的影响,为建立美国白蛾室内繁育体系提供依据。【方法】将人工饲料组分严格定量,并按2%和4%的质量分数分别添加葡萄糖、海藻糖和蔗糖(即6个处理),以其饲喂美国白蛾4龄幼虫,试验期间测定幼虫体质量、蜕皮率、死亡率及体内蛋白含量和3种消化酶(α-淀粉酶、脂肪酶和胰蛋白酶)活性。【结果】2%葡萄糖对美国白蛾幼虫生长发育有一定的促进作用。2%蔗糖处理组的幼虫体内蛋白含量随处理时间延长呈先上升后稳定的趋势,而其他处理组的蛋白含量基本呈下降趋势。6个处理组的幼虫体内α-淀粉酶活性均随处理时间的延长呈上升趋势;随着处理时间的延长,不同含量葡萄糖和海藻糖处理组的脂肪酶和胰蛋白酶活性均呈下降趋势,4%蔗糖处理组的这两种酶活性也呈下降趋势,但下降幅度较小,而2%蔗糖处理组的胰蛋白酶活性呈现上升趋势,脂肪酶活性呈先下降后上升趋势。【结论】相比于葡萄糖和海藻糖,蔗糖更适宜作为美国白蛾人工饲料的糖类成分。【Objective】The impact of carbohydrates on the growth,development and digestive enzyme activity of Hyphantria cunea(American white moth)was investigated,aiming to provide a scientific foundation for establishing an indoor breeding system.【Method】Strictly quantified artificial feed,supplemented with glucose,trehalose and sucrose at 2%and 4%mass fractions,which was summed up to 6 treatment groups,was fed to the 4th instar larvae of American white moth.During the experiment,the body mass,molting rate,mortality and protein content of larvae,as well as the activities of 3 digestive enzymes(α-amylase,lipase and trypsin)were measured.【Result】Supplementation of 2%glucose exerted certain promoting effect on the growth and development of American white moth larvae.The protein content of larvae supplemented with 2%sucrose started with an uprise and then stabilized along the experiment,while others followed decreasing trends.α-amylase activity in the larvae of all the 6 treatment groups showed increasing trends with the experiment,while the activities of lipase and trypsin were declined in the groups treated with glucose and trehalose at both rates.In the treatment added 4%sucrose,glucose and trehalose activities experinced very subtle declines;while in the treatment added 2%sucrose,trypsin activity followed an uptrend,and lipase activity was firstly decreased and then increased.【Conclusion】Sucrose is the most suitable carbohydrate component for the artificial feed of American white moth in comparison to glucose and trehalose.

关 键 词：葡萄糖 海藻糖 蔗糖 生长发育 消化酶 美国白蛾 室内繁育 

分 类 号：S763.3[农业科学—森林保护学]