短期低温胁迫对油茶叶片非结构性碳水化合物的影响  

Effect of short-term low temperature stress on non-structural carbohydrates in Camellia oleifera leaves

作　　者：王红润 夏建梅 孙永江 WANG Hongrun;XIA Jianmei;SUN Yongjiang(State Key Laboratory of Efficient Production of Forest Resources/College of Forestry,Beijing Forestry University,Beijing 100000,China;Xinxian Lingrui Oil Tea Research Institute,Xinyang,Henan 464000,China)

机构地区：[1]林木资源高效生产全国重点实验室/北京林业大学林学院,北京100000 [2]河南新县羚锐油茶研究院,河南信阳464000

出　　处：《福建农林大学学报(自然科学版)》2025年第3期392-400,共9页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(32001348)。

摘　　要：【目的】探讨低温胁迫下油茶(Camellia oleifera)叶片的非结构性碳水化合物(NSC)含量及其代谢机制,为提高油茶抗寒性和优化种植策略提供理论依据。【方法】以2年生‘长林40号’油茶为试验材料,对其进行低温(0、-5℃)和常温(对照)6、12、18、24 h处理,观察表型,并测定可溶性糖、淀粉等NSC的含量,以及蔗糖代谢酶活性,分析短期低温胁迫对油茶叶片NSC的影响。【结果】低温胁迫导致油茶叶片的丙二醛(MDA)和过氧化氢(H_(2)O_(2))浓度增加,且随着胁迫程度的加剧,二者的增加幅度增大。与对照相比,低温胁迫导致油茶叶片淀粉含量减少,可溶性糖含量增加;低温胁迫下蔗糖合成酶(SS)活性和蔗糖磷酸合成酶(SPS)活性有所降低,酸性转化酶(AI)和中性转化酶(NI)活性升高。【结论】低温胁迫对油茶叶片NSC的含量有明显影响,油茶通过提高AI和NI活性促进葡萄糖和果糖含量增加,进而调节可溶性糖及淀粉含量以应对低温胁迫。【Objective】The contents and metabolic mechanism of non-structural carbohydrates(NSC)in Camellia oleifera leaves under low temperature stress were investigated to provided theoretical basis for improving cold resistance and optimizing planting strategy of C.oleifera.【Method】Different low temperature treatments(0 and-5℃,with room temperature as the control)for 6,12,18,and 24 hours were applied on C.oleifera'Changlin 40’for the analyses on phenotype,contents of NSC(namely soluble sugars and starch),as well as activities of sucrose metabolizing enzymes,to analyze the effect of short-term low temperature stress on NSC levels in C.oleifera leaves.【Result】Low temperature stress led to increases in the concentrations of malondialdehyde(MDA)and H_(2)O_(2) in C.oleifera leaves,and the increasing rates were enhanced with the severity of stress.Low temperature stress resulted in lower contents of starch while higher contents of soluble sugar in C.oleifera leaves;at the same time,the activities of sucrose synthase and sucrose phosphate synthase declined compared with the control group;while the activities of acid invetase and neutral invetase went up.【Conclusion】Low temperature stress exerted significant impacts on the contents of NSC in C.oleifera leaves;the adaptability of C.oleifera to low temperature was achieved by enhancing the activities of acid invetase and neutral invetase,which led to greater contents of soluble sugar(glucose and fructose)and starch level.

关 键 词：短期低温胁迫 油茶 非结构性碳水化合物 糖代谢 蔗糖 

分 类 号：S791[农业科学—林木遗传育种]