耐冷节杆菌Csp1基因提高拟南芥抗寒能力  

Cold tolerance of Arabidopsis thaliana enhanced by Csp1 gene of Arthrobacter sp.C2

作　　者：吴林轩 兰依航 姜涵奕 马添 李春艳[1] 臧海莲[1] WU Linxuan;LAN Yihang;JIANG Hanyi;MA Tian;LI Chunyan;ZANG Hailian(School of Resources and Environment,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《东北农业大学学报》2025年第1期50-60,69,共12页Journal of Northeast Agricultural University

基　　金：国家自然科学基金面上项目(42071268)。

摘　　要：提升植物抗寒能力是克服低温对植物生长限制的关键。冷休克蛋白(CSPs)是来源于生物体的一类高度保守的核酸结合蛋白,参与细胞内基因复制、转录和翻译过程,以及蛋白折叠、膜流动和非生物胁迫应答等活动。这类蛋白可增强植物对寒冷、干旱和盐碱的耐受能力。研究从耐冷节杆菌(Arthrobacter sp.)C2基因组DNA中克隆出Csp1基因,构建植物表达载体pBI121-Csp1,通过花序浸染法将其转化至拟南芥,在低温条件下观察植株表型,检测抗氧化酶活性和与耐冷相关基因的表达水平,以探究Csp1基因提高拟南芥抗寒能力机制。结果表明:低温胁迫条件下,过表达Csp1基因拟南芥存活率显著高于野生型;拟南芥叶片丙二醛(MDA)水平及离子渗透率低于野生型;超氧化物歧化酶(SOD)、过氧化氢(CAT)和过氧化物酶(POD)活性均高于野生型拟南芥;过表达Csp1基因可提高拟南芥低温胁迫相关基因AtCBF1、AtCBF3、AtCOR15a、AtCOR47、AtRD29a、AtKIN1、AtMnSOD、AtCAT、AtPOD及Arthrobacter sp.C2菌中Csp1表达水平。综上所述,通过过表达Csp1基因可增强拟南芥抗寒能力。研究为提升植物对低温胁迫适应能力、培育抗寒植物新品种提供参考。How to improve the cold resistance of plants is the key problem to overcome the restriction of low temperature on plant growth.Cold shock proteins(CSPs),a class of highly conserved nucleic acid-binding proteins derived from organisms,are involved in the replication,transcription and translation of intracellular genes,as well as protein folding,membrane flow and abiotic stress response.In addition,these proteins could enhance plant tolerance to cold,drought and salt stresses.In this study,Csp1 gene was cloned from Arthrobacter sp.C2 genomic DNA and plant expression vector pBI121-Csp1 was constructed,which was transformed into Arabidopsis thaliana using the floral dip method.The plant phenotype was observed,and the activity of antioxidase and expression levels of genes related to cold tolerance were detected under low temperature to analyze the mechanism of cold tolerance conferred by Csp1 gene.The results showed that the survival rate of Arabidopsis thaliana overexpressing Csp1 gene was significantly higher than that of wild type under low temperature stress.The levels of malondialdehyde(MDA)and ion leakage in Arabidopsis thaliana leaves was lower than that of wild type.The activities of superoxide dismutase(SOD),hydrogen peroxide(CAT)and peroxidase(POD)in Arabidopsis thaliana overexpressing Csp1 gene were higher than those of wild type.Overexpression of Csp1 gene could increase the expression levels of AtCBF1,AtCBF3,AtCOR15a,AtCOR47,AtRD29a,AtKIN1,AtMnSOD,AtCAT,AtPOD and Csp1(from Arthrobacter sp.C2)genes related to low temperature stress in Arabidopsis thaliana.The findings suggested that overexpression of Csp1 gene could enhance the cold resistance of Arabidopsis thaliana providing an important reference for improving plant adaptability to low temperature stress and breeding new varieties of cold resistant plants.

关 键 词：节杆菌 Csp1基因 拟南芥 低温胁迫 抗寒能力 

分 类 号：S332.5[农业科学—作物遗传育种]