刺五加复合诱变效应分析及诱变体系的构建  

Effects of Eleutherococcus Senticosus Maxim and Construction of Mutagenesis System

作　　者：斯琴图雅 汤明威 张丹丹 孙培琳 张子呈 由香玲[2] 高娃 Siqin Tuya;Tang Mingwei;Zhang Dandan;Sun Peilin;Zhang Zicheng;You Xiangling;Gao Wa(Heilongjiang Institute of Atomic Energy,Harbin 150086,China;College of Life Sciences,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]黑龙江省原子能研究院,哈尔滨150086 [2]东北林业大学生命科学学院,哈尔滨150040

出　　处：《黑龙江科学》2025年第8期96-99,共4页Heilongjiang Science

基　　金：黑龙江省科学院院长基金项目(YZ2023YZN01);黑龙江省省属科研院所科研业务费项目(CZKYF2023-1A007)。

摘　　要：为探明γ射线和电子束复合诱变技术的协同效应,确定刺五加沙藏萌动种子的最优复合诱变剂量,采用L_(25)(3~5)正交试验设计,系统评估了γ射线和电子束对刺五加沙藏萌动种子相对出苗率的影响。研究结果表明,γ射线和电子束对刺五加沙藏萌动种子相对出苗率的影响均为极显著(P<0.01);γ射线单因素诱变的半致死剂量为32.1 Gy,电子束单因素诱变的半致死剂量为34.7 Gy。复合诱变的协同效应对刺五加沙藏萌动种子相对出苗率的影响为显著(P<0.05),适宜的复合诱变剂量为10 Gy(γ射线)+20 Gy(电子束)。此研究为复合诱变育种技术方法的进一步研究和应用提供了重要参考。To elucidate the synergistic effects of combined gamma ray and electron beam mutagenesis and to determine the optimal combined mutagenic dose for the germination of E.senticosus seeds in stratification,a systematic investigation is conducted using the L 25(35)orthogonal experimental design,and the impact of gamma rays and electron beams on the relative germination rate of stratified E.senticosus seeds is assessed systematically.The results indicate that the influence of gamma rays and electron beams on the relative germination rate of stratified E.senticosus seeds is highly significant(P<0.01).LD 50 for gamma ray single-factor mutagenesis is 32.1 Gy,and for electron beam single-factor mutagenesis is 34.7 Gy.The synergistic effect of combined gamma ray and electron beam mutagenesis on the relative germination rate of stratified E.senticosus seeds is also significant(P<0.05),with the appropriate combined mutagenic dose being 10 Gy(gamma rays)+20 Gy(electron beams).The study provides important reference for the further research and application of combined mutagenesis breeding techniques.

关 键 词：刺五加 复合诱变 Γ射线 电子束 正交试验 

分 类 号：S567.19[农业科学—中草药栽培]