浓缩生长因子促进人微血管内皮细胞血管形成的作用及机制研究  

Study of the angiogenic effects and mechanisms of concentrated growth factor on human microvascular endothelial cells

作　　者：张佳佳 尚圣兰 刘万兵 李涛 ZHANG Jiajia;SHANG Shenglan;LIU Wanbing;LI Tao(Department of Endocrinology,General Hospital of Central Theater Command,Wuhan,Hubei 430070,China;Department of Clinical Pharmacology,General Hospital of Central Theater Command,Wuhan,Hubei 430070,China;Department of Transfusion Medicine,General Hospital of Central Theater Command,Wuhan,Hubei 430070,China)

机构地区：[1]中部战区总医院内分泌科,武汉430070 [2]中部战区总医院临床药学科,武汉430070 [3]中部战区总医院输血科,武汉430070

出　　处：《重庆医学》2025年第4期801-805,812,共6页Chongqing Medical Journal

基　　金：国家自然科学基金青年科学基金项目(82000776);中部战区总医院育英计划项目(ZZY202202)。

摘　　要：目的探讨浓缩生长因子(CGF)对人微血管内皮细胞(HMEC-1)血管形成的影响及相关机制。方法将HMEC-1细胞分为Control组、CGF组、CGF+LY294002组,采用细胞计数试剂盒(CCK-8)、Transwell实验及成管实验检测并比较各组细胞的增殖、迁移、小管形成能力,Western blot检测并比较各组细胞内磷酸肌醇-3-激酶(PI3K)、磷酸化-PI3K(p-PI3K)、蛋白激酶B(Akt)、磷酸化-Akt(p-Akt)、哺乳动物雷帕霉素靶蛋白(mTOR)及磷酸化-mTOR(p-mTOR)表达水平。结果与Control组比较,CGF组细胞增殖、迁移及成管能力提升(P<0.05),且细胞内p-PI3K、p-Akt及p-mTOR表达水平增加(P<0.05);与CGF组比较,CGF+LY294002组细胞增殖、迁移及成管能力降低(P<0.05),细胞内p-PI3K、p-Akt及p-mTOR表达水平减少(P<0.05)。结论CGF可以通过PI3K/mTOR/Akt通路促进HMEC-1细胞的增殖、迁移及血管形成。Objective To investigate the effect of concentrated growth factor(CGF)on angiogenesis in human microvascular endothelial cells(HMEC-1)and explore the underlying mechanisms.Methods HMEC-1 were divided into the control group,CGF group,and CGF+LY294002 group.Cell proliferation,migration,and tubule formation abilities were measured and compared using the Cell Counting Kit-8(CCK-8),Transwell assay,and tube formation assay,respectively.The protein expression levels of phosphoinositide 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),phospho-protein kinase B(Akt),phosphorylated Akt(p-Akt),mammalian target of rapamycin(mTOR),and phosphorylated mTOR(p-mTOR)were detected and compared by Western blot.Results Compared with the control group,the CGF group exhibited enhanced cell proliferation,migration,and tubule formation abilities(P<0.05),along with increased expression levels of p-PI3K,p-Akt,and p-mTOR(P<0.05).Compared with the CGF group,the CGF+LY294002 group demonstrated reduced cell proliferation,migration,and tubule formation abilities(P<0.05),accompanied by decreased expression levels of p-PI3K,p-Akt,and p-mTOR(P<0.05).Conclusion CGF promotes the proliferation,migration,and angiogenesis of HMEC-1 via the PI3K/mTOR/Akt pathway.

关 键 词：浓缩生长因子 人微血管内皮细胞 血管生成 磷酸肌醇3激酶/蛋白激酶B/哺乳动物里帕霉素靶蛋白通路 

分 类 号：R641[医药卫生—外科学]