糖尿病下骨髓细胞的炎症特点  

Inflammatory Characteristics of Bone Marrow Cells in Diabetes

作　　者：赵继伟 董圆 邓涯兰 颜岑 杨暄一 冯英梅 ZHAO Ji-wei;DONG Yuan;DENG Ya-lan;YAN Cen;YANG Xuan-yi;FENG Ying-mei(Beijing Youan Hospital,Capital Medical University,Beijing,100069,China;Basic Clinical Joint Laboratory for Research on Viral Hepatitis Related Liver Diseases,Capital Medical University,Beijing,100069,China)

机构地区：[1]首都医科大学附属北京佑安医院,北京100069 [2]首都医科大学病毒性肝炎相关肝病研究基础临床联合实验室,北京100069

出　　处：《现代生物医学进展》2025年第7期1121-1131,1198,共12页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(82070841)。

摘　　要：目的:探讨糖尿病状态下,骨髓细胞内的炎症与相关因子表达的变化特点。方法:首先通过分析24周龄的Db/db小鼠和同周龄Db/m小鼠的血糖、白细胞亚群以及对白细胞内各成分定量分析等,来验证糖尿病小鼠模型。使用QAM-INF-1芯片,通过Quantibody mouse炎症阵列方法检测8周龄和24周龄的Db/db小鼠骨髓细胞中的炎症因子表达水平,并通过GO富集分析来了解其在细胞组成、生物过程和分子功能方面的作用,以及将差异表达基因进行KEGG富集分析,把差异显著的通路进行富集,找到显著性差异变化的生物学调控通路,探讨在糖尿病小鼠的病程长短与骨髓细胞内的炎症状态变化的关系。结果:空腹血糖的检测结果表示,Db/db小鼠的血糖水平显著高于Db/m小鼠;通过全血血常规对血液细胞分类及计数,Db/db小鼠的血液中的白细胞数、粒细胞数、粒细胞百分比均高于Db/m小鼠;与8周龄的糖尿病小鼠相比,在24周龄的糖尿病小鼠骨髓细胞内,单核细胞趋化蛋白-1(MCP-1)、金属肽酶抑制因子-1(TIMP-1)的表达降低,趋化因子配体5(RANTES)、化学趋化因子(CXCL1)、肿瘤坏死因子受体-2(TNFR2)的表达增高。通过GO数据库和KEGG富集分析对筛选出的基因进行了功能注释和通路注释,并用统计方法计算差异基因的富集程度,确定了它们参与的代谢和信号通路。结论:随着糖尿病病程的延长,骨髓细胞的炎症状态逐渐加剧,骨髓细胞内MCP-1和TIMP-1的表达降低,CXCL1、RANTES、TNFR2的表达增高。Objective:To investigate the changes of inflammation and related factors expression in bone marrow cells in diabetes.Methods:Fasting blood glucose levels,leukocyte subsets and quantitative analy sis of various components in leukocytes of 24-week-old Db/db mice and the same week-old Db/m mice were analyzed to verify the diabetes mouse model.Then the quantitative mouse inflammatory array method was used to detect the expression level of inflammatory factors in the bone marrow cells of8-week-old and 24-week-old Db/db mice,and the GO enrichment analysis was used to understand its role in cell composition,biological process and molecular function,as well as KEGG enrichment analysis of differentially expressed genes.The significantly different pathways were enriched to find the biological regulation pathway of significantly different changes,and explore the relationship between the duration of disease in diabetes mice and the changes of inflammatory status in bone marrow cells.Results:The results of fasting blood glucose testing showed that the blood glucose levels in Db/db mice were significantly higher than those in Db/m mice;By classifying and counting blood cells through whole blood routine,the white blood cell count,granulocyte count,and granulocyte percentage in the blood of Db/db mice were higher than those of Db/m mice;Compared with 8-week-old diabetes mice,the expression of monocyte chemoattractant protein-1( MCP-1) and metallopeptidase inhibitor protein-1( TIMP-1) in bone marrow cells of 24-week-old diabetes mice decreased,and the expression of chemokine ligand 5( RANTES),chemotactic factor( CXCL1),and tumor necrosis factor receptor 2( TNFR 2) increased.The screened genes were functionally annotated and pathway annotated through GO database and KEGG enrichment analysis,and the enrichment degree of differentially expressed genes was calculated using statistical methods to determine their involvement in metabolism and signaling pathways.Conclusions:Along with the diabetic progression,the inflammatory state of b

关 键 词：糖尿病 骨髓细胞 炎症 血糖 细胞因子 

分 类 号：R3[医药卫生—基础医学] R-335[生物学—生物工程] Q813R364.5