玉米醇溶蛋白基芦丁颗粒的构建、表征及稳定性分析  

Preparation,characterization,and stability analysis of zein-based rutin nanoparticles

作　　者：周雨晨 党志雄 蔡佳乐 杨芳[1] ZHOU Yuchen;DANG Zhixiong;CAI Jiale;YANG Fang(School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Key Laboratory of Green Chemical Process of Ministry of Education(Wuhan Institute of technology),Engineering Research Center of Phosphorus Resources Development and Utilization of Ministry of Education,Hubei Key Laboratory of Novel Reactor and Green Chemical Technology,Wuhan 430205,China)

机构地区：[1]武汉工程大学环境与生态工程学院,绿色化工过程教育部重点实验室(武汉工程大学),国家磷资源开发利用工程技术研究中心,新型反应器与绿色化学工艺湖北省重点实验室,湖北武汉430205

出　　处：《武汉工程大学学报》2025年第2期187-193,共7页Journal of Wuhan Institute of Technology

基　　金：国家自然科学基金(31701604);磷资源开发利用教育部工程研究中心开放/创新基金(GCP202103);绿色化工过程教育部重点实验室开放项目(GCP202103)。

摘　　要：为了提高芦丁的生物利用率,以玉米醇溶蛋白为载体采用反溶剂法制备包载芦丁的玉米醇溶蛋白纳米颗粒,并对其进行表征。研究结果表明:采用不同的玉米醇溶蛋白和芦丁质量比制备的纳米颗粒粒径都小于200 nm;当玉米醇溶蛋白与芦丁质量比为7∶1时,芦丁的包载率达到最高(50.50%);Docking分子对接和红外光谱分析表明,玉米醇溶蛋白与芦丁结合的主要驱动力是氢键、静电和疏水作用力。此外,玉米醇溶蛋白芦丁纳米颗粒在远离等电点和高盐时稳定性较好。To improve the bioavailability of rutin,the antisolvent precipitation method was employed to prepare zein nanoparticles encapsulatin with rutin,and the properties of nanoparticles were characterized and analyzed.The results showed that the average particle sizes of all nanoparticles with different mass ratio of zein to rutin were under 200 nm in diameter.When the mass ratio of zein to rutin was 7∶1,the encapsulation efficiency of rutin reached the highest level(50.50%).Notably,both molecules docking analysis and Fourier transform infrared spectrometry confirmed that rutin and zein might interact through hydrogen bonding,electrostatic and hydrophobic interactions.Moreover,the stability of the nanoparticles was significantly enhanced when they were kept away from the isoelectric point and high-salt environments.

关 键 词：反溶剂法 芦丁 玉米醇溶蛋白 纳米颗粒 表征 

分 类 号：TQ460[化学工程—制药化工]