外泌体在生物标志物研究领域的文献计量学分析  

Bibliometric analysis of exosomes in the biomarker research field

作　　者：黄雨霞 王海燕 张屹涵 蔺奕菲 乔晓强[2] 胡良海[1] HUANG Yuxia;WANG Haiyan;ZHANG Yihan;LIN Yifei;QIAO Xiaoqiang;HU Lianghai(School of Life Sciences,Jilin University,Changchun 130012,China;College of Pharmaceutical Sciences,Hebei University,Hebei 071002,China)

机构地区：[1]吉林大学生命科学学院,吉林长春130012 [2]河北大学药学院,河北保定071002

出　　处：《色谱》2025年第5期498-507,共10页Chinese Journal of Chromatography

基　　金：国家自然科学基金(22274035,22374056).

摘　　要：外泌体是细胞分泌的细胞外囊泡的一种亚型,携带丰富的遗传物质及蛋白质等,外泌体膜表面有众多母细胞特有的标志物,已成为疾病诊断、疾病进展以及疾病治疗的重要依据。本文在Web of Science core collection(SCI-EXPENED)数据库中,检索2010-2024年间研究主题为“外泌体”与“生物标志物”或“诊断”或“液体活检”的研究型和综述类论文。文献计量分析结果显示,外泌体作为生物标志物标记疾病的研究关注度逐渐升高,中国是该研究的主要贡献国家;文献主要围绕癌症、炎症、糖尿病、神经退行性疾病和心血管疾病的标记诊断展开研究;通常使用色谱、质谱、拉曼光谱等技术分析外泌体核酸、蛋白质、代谢物等;常用的检验样本为血浆、血清、尿液、唾液、脑脊液等体液;外泌体作为肿瘤标志物的研究主要聚焦于肺癌、乳腺癌、前列腺癌等8种具有高发病率的癌症。本文聚焦于外泌体作为疾病生物标志物的研究领域,利用文献计量工具系统分析了2010-2024年间外泌体及其内容物作为生物标志物在疾病诊断领域中的应用,并对外泌体介导的疾病诊断和疾病治疗的发展前景和未来努力方向进行了分析和展望,期望为外泌体在疾病标志及应用方面的研究提供参考。Exosomes are extracellular vesicles secreted by cells and are rich in genetic material and proteins.The surfaces of exosome membranes contain many blast-specific markers that provide an important basis for disease diagnosis,progression,and treatment.Herein,we searched the Web of Science core collection(SCI-EXPENED)database for research and review articles on“exosomes”and“biomarkers”or“diagnostics”or“liquid biopsy”as research topics between 2010 and 2024.Bibliometric analysis revealed that exosomes have received increasing levels of attention as disease biomarkers,with China contributing the most to these studies.Herein,we focus on marker diagnoses for cancer,inflammation,and diabetes,as well as neurodegenerative and cardiovascular diseases.Chromatography,mass spectrometry,Raman spectroscopy,and other techniques are typically used to analyze exosomal nucleic acids,proteins,and metabolites,with commonly used test samples including plasma,serum,urine,saliva,cerebrospinal fluid,and other bodily fluids.Research into exosomes as tumor markers has mainly focused on eight highly prevalent cancers,including lung,breast,and prostate cancers.This paper focuses on exosomes as disease biomarkers and uses a bibliometric tool system to analyze the use of exosomes and their contents as biomarkers in the disease diagnosis field between 2010 and 2024,analyzes development prospects,and discusses future exosome-mediated efforts for diagnosing and treating diseases,and is expected to provide a reference for studying and applying exosomes as disease markers.

关 键 词：外泌体 生物标志物 液体活检 疾病诊断 文献计量学分析 

分 类 号：O658[理学—分析化学]