基于16S核糖体核糖核酸测序技术的睡眠呼吸暂停儿童扁桃体和腺样体组织细菌群落分析  

Microbiota profiling of tonsil and adenoid tissues in children with sleep apnea using 16S rRNA sequencing

作　　者：刘丽洋 宋晓蕊 僧东杰 段清川 张杰 苏钰[3] 许莹 LIU Liyang;SONG Xiaorui;SENG Dongjie;DUAN Qingchuan;ZHANG Jie;SU Yu;XU Ying(Department of Otorhinolaryngology Head and Neck Surgery,Henan Children's Hospital,Zhengzhou 450003,China)

机构地区：[1]河南省儿童医院耳鼻咽喉头颈外科,郑州450003 [2]河南省儿童医院儿童感染性疾病研究室,郑州450003 [3]解放军总医院第二医学中心耳鼻咽喉头颈外科,北京100039 [4]首都医科大学附属北京儿童医院耳鼻咽喉头颈外科,100045

出　　处：《传染病信息》2025年第2期160-166,共7页Infectious Disease Information

基　　金：国家自然科学基金项目(82000850)。

摘　　要：目的分析儿童阻塞性睡眠呼吸暂停(obstructivesleepapnea,OSA)相关扁桃体及腺样体组织的菌群组成。方法将2023年1一5月在河南省儿童医院经睡眠呼吸监测确诊OSA并行扁桃体及腺样体切除术的患儿15例纳入研究,其中8例单纯扁桃体肥大患儿归为非炎症组,7例既往病史存在扁桃体反复发炎患儿归为炎症组。术前收集腺样体近鼻咽部表面、腺样体近口咽部表面、左扁桃体表面及右扁桃体表面黏膜拭子标本,手术切除后收集左扁桃体外层组织、右扁桃体外侧组织、左扁桃体核心组织及右扁桃体核心组织标本,应用16S核糖体核糖核酸测序技术分析样本的细菌组成。结果腺样体不同取样部位细菌多样性和群落组成基本相同;扁桃体表面与核心组织的细菌群落组成以及群落的丰度指数Ace和Chaol差异存在统计学意义(P均<0.05);炎症组与非炎症组之间的普雷沃氏菌属、梭杆菌属、韦荣氏菌属、链球菌属和奈瑟氏菌属的相对丰度差异存在统计学意义(P均<0.05)。结论可利用腺样体表面菌落特征评估整个腺体细菌组成。扁桃体不同部位的细菌多样性和组成存在差异,儿童OSA可能是多种共生或致病细菌及其免疫反应的结果,而不是组织或表面的单个细菌所致。Objective To investigate the flora composition of tonsils and adenoids associated with obstructive sleep apnea in children.Methods Fifteen children diagnosed with sleep apnea monitoring and treated with tonsillectomy and adenoidectomy were included in this study.Among these,8 patients with isolated tonsillar hypertrophy were assigned to the non-inflammatory group.while 7 patients with a history of recurrent tonsillar inflammation were allocated to the inflammatory group.Preoperative adenoidal nasopharyngeal swabs,proximal pharyngeal swabs and bilateral tonsillar surface swabs were collected,bilateral tonsillar tissue and core tissue specimens were collected after surgical resection,and the bacterial composition of each specimen was analyzed by 16S ribosomal RNA-encoding gene sequencing technology.Results The bacterial diversity and community composition of adenoids were basically the same at different sampling sites.There were significant differences in the composition of microbial communities and the abundance indexes of Ace and Chao1 between the tonsil surface swabs and the core tissues(P<0.05).There were also significant differences in the relative abundance of Prevonella,Fusobacterium,Veronella,Streptococcus,and Neisseria between the inflammatory and non-inflammatory groups(P<0.05).Conclusion The colony characteristics of the adenoid surface swabs can be used to assess overall adenoid microbial composition;There are differences in bacterial diversity and composition in the tonsils,indicating that OSA in children may be the result of multiple commensal or pathogenic bacteria and their associated immune responses,rather than being attributable to individual bacteria present on the tissue or surface.

关 键 词：人体微生态 腺样体 扁桃体 16SrRNA测序 

分 类 号：R766[医药卫生—耳鼻咽喉科]