黄芩素靶向调控CD47对喉癌细胞凋亡、免疫逃逸的影响  

Effects of baicalein targeting CD47 on apoptosis and immune escape of laryngeal carcinoma cells

作　　者：高树峰[1] 王心涛[1] 游龙贵[1] 黄芸 潘其彬 卢晓华 GAO Shu-feng;WANG Xin-tao;YOU Long-gui;HUANG Yun;PAN Qi-bin;LU Xiao-hua(Department of Otolaryngology Head and Neck Surgery,Ganzhou People's Hospital,Ganzhou 341000,Jiangxi Province,China;Departmentof Otolaryngology,Ganzhou Fifth People's Hospital,Ganzhou 341000,Jiangxi Province,China)

机构地区：[1]赣州市人民医院耳鼻咽喉头颈外科,江西赣州341000 [2]赣州市第五人民医院五官科,江西赣州341000

出　　处：《中国临床药理学杂志》2025年第3期320-324,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨黄芩素对喉癌细胞凋亡、免疫逃逸的影响及作用机制。方法将人喉癌细胞TU686细胞随机分为对照组(常规培养)、黄芩素组(50μmol·L^(-1)黄芩素)、Vector组(转染Vector+50μmol·L^(-1)的黄芩素)、CD47组[转染分化簇47(CD47)+50μmol·L^(-1)的黄芩素]。用5-乙炔基-2′-脱氧尿苷(Edu)法、Annexin V-FITC/PI双染法检测分别检测细胞增殖、凋亡情况,用蛋白质印迹法检测增殖、凋亡蛋白表达情况;并将人外周淋巴细胞分别与各组细胞共培养,检测淋巴细胞对TU686细胞杀伤率,观察TU686细胞的被吞噬率。结果对照组、黄芩素组、Vector组、CD47组细胞Edu阳性细胞率分别为(36.24±2.74)%、(13.33±1.93)%、(14.22±1.48)%和(27.80±2.74)%,凋亡率分别为(3.94±0.40)%、(28.40±1.76)%、(26.60±1.41)%和(12.33±0.84)%,CD47蛋白相对表达水平分别为1.00±0.11、0.42±0.07、0.44±0.06和1.21±0.12,细胞周期素D1(CyclinD1)蛋白相对表达水平分别为0.86±0.08、0.37±0.05、0.36±0.04和0.74±0.08,裂解的半胱氨酸天冬氨酸蛋白酶-7(Cl-Caspase-7)蛋白相对表达水平分别为0.35±0.04、0.99±0.12、1.00±0.11和0.50±0.06,人外周淋巴细胞对TU686细胞的杀伤率(1∶1比例共培养)分别为(10.61±1.19)%、(32.02±2.27)%、(31.86±2.06)%和(14.39±1.07)%,细胞吞噬率分别为(6.35±0.71)%、(11.99±1.64)%、(12.03±0.76)%和(7.06±0.57)%,黄芩素组的上述指标与对照组比较,CD47组的上述指标与Vector组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01,P<0.001)。结论黄芩素可能通过抑制CD47表达诱导喉癌细胞凋亡、抑制喉癌细胞免疫逃逸,进而减缓喉癌进展。Objective To investigate the effects of baicalein on apoptosis and immune escape of laryngeal carcinoma cells and its mechanism.Methods Human laryngeal carcinoma TU686 cells were randomly divided into control group(routine culture),baicalein group(50μmol·L^(-1)baicalein),Vector group(transfected with Vector+50μmol·L^(-1)baicalein)and CD47 group[transfected with differentiation cluster 47(CD47)+50μmol·L^(-1)baicalein].Cell proliferation and apoptosis were detected by 5-acetylidene-2′-deoxyuridine(Edu)method and Annexin V-FITC/PI double staining method.The expression of proliferative and apoptotic proteins were detected by Western blot.Human peripheral lymphocytes were co-cultured with cells in each group to detect the killing rate of TU686 cells.The phagocytosis rate of TU686 cells was observed.Results The Edu positive cell rates in control group,baicalein group,Vector group and CD47 group were(36.24±2.74)%,(13.33±1.93)%,(14.22±1.48)%and(27.80±2.74)%,respectively;the apoptosis rates were(3.94±0.40)%,(28.40±1.76)%,(26.60±1.41)%and(12.33±0.84)%,respectively;the relative expressions of CD47 protein were 1.00±0.11,0.42±0.07,0.44±0.06 and 1.21±0.12,respectively;the relative expression levels of CyclinDl protein were 0.86±0.08,0.37±0.05,0.36±0.04 and 0.74±0.08,respectively;the relative expression levels of cleaved cysteine aspartate proteinase-7(Cl-Caspase-7)protein were0.35±0.04,0.99±0.12,1.00±0.11 and 0.50±0.06,respectively;the killing rates of human peripheral lymphocytes to TU686 cells(1:1 ratio)were(10.61±1.19)%,(32.02±2.27)%,(31.86±2.06)%and(14.39±1.07)%,respectively;the phagocytosis rates were(6.35±0.71)%,(11.99±1.64)%,(12.03±0.76)%and(7.06±0.57)%,respectively.The above indicators,baicalein group compared with the control group,Vector group compared with CD47 group,the differences were statistically significant(P<0.05,P<0.01,P<0.001).Conclusion Baicalein may induce the apoptosis of laryngeal carcinoma cells and inhibit the immune escape of laryngeal carcinoma cells by inhibitin

关 键 词：黄芩素 喉癌 细胞凋亡 免疫逃逸 分化簇47 

分 类 号：R28[医药卫生—中药学]