LINC02147抑制槟榔碱诱导的人口腔黏膜成纤维细胞恶性进展的研究  

LINC02147 inhibit the malignant progression of human oral mucosal fibroblasts induced by arecoline

作　　者：肖青锋 邴小三 XIAO Qing-feng;BING Xiao-san(Department of Stomatology,Affliated Hospital of Hubei University of Arts and Sciences,Xiangyang Central Hospital,Xiangyang 441000,Hubei Province,China;Department of Pediatrics,Affliated Hospital of Hubei University of Arts and Sciences,Xiangyang Central Hospital,Xiangyang 441000,Hubei Province,China)

机构地区：[1]湖北文理学院附属医院,襄阳市中心医院口腔科,湖北襄阳441000 [2]湖北文理学院附属医院,襄阳市中心医院儿科,湖北襄阳441000

出　　处：《中国临床药理学杂志》2025年第3期325-329,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究长链非编码RNA(lncRNA)LINC00922调控分泌型卷曲相关蛋白1(SFRP1)抑制槟榔碱诱导的人口腔黏膜成纤维(hOMF)细胞恶性进展。方法hOMF细胞分为空白对照(NC)组(hOMF细胞不做任何处理)、模型组(60 mg·L^(-1)槟榔碱处理)、过表达LINC02147(oe-LINC02147)组(60 mg·L^(-1)槟榔碱处理,转染过表达LINC02147载体)、小干扰SFRP1(si-SFRP1)组(60 mg·L^(-1)槟榔碱处理,转染过表达LINC02147载体、转染SFRP1抑制药载体)。用实时荧光定量聚合酶链反应检测各组细胞SFRP1 mRNA相对表达水平,用免疫荧光检测各组细胞中α-平滑肌肌动蛋白(α-SMA)及vimentin阳性表达,用5-乙炔-2′-脱氧尿苷检测各组细胞增殖情况,用二氯二氢荧光素醋酯荧光探针法检测各组细胞中活性氧化物种(ROS)含量。结果模型组和oe-LINC02147组SERP1 mRNA相对表达水平分别为1.00±0.08和3.75±0.59;空白对照组、模型组、oe-LINC02147组和si-SFRP1组的细胞增殖率分别为(43.61±6.97)%、(12.98±4.27)%、(38.66±5.12)%和(15.73±5.42)%,ROS含量分别为(9.42±1.73)%、(36.58±6.16)%、(12.89±2.08)%和(33.45±5.76)%,α-SMA阳性表达分别为1.00±0.13、5.02±1.46、2.36±0.48和4.37±0.97,vimentin阳性表达分别为1.00±0.15、4.63±1.51、1.98±0.85和3.92±1.36。模型组的上述指标与空白对照组比较、oe-LINC02147组的上述指标与模型组比较,si-SFRP1组的上述指标与oe-LINC02147组比较,在统计学上差异均有统计学意义(均P<0.001)。结论LINC02147可能通过调控SFRP1来抑制槟榔碱诱导的hOMF细胞恶性进展。Objective Study on the long non-coding RNA(lncRNA)LINC00922 regulating secreted frizzled related protein 1(SFRP1)to inhibit the malignant progression of human oral mucosal fibroblasts(hOMF)induced by arecoline.Methods Cells were divided into blank control(NC)group(hOMF cells were not treated in any way),model group(60 mg·L^(-1)arecoline treatment),oe-LINC02147 group(60 mg·L^(-1)arecoline treatment,transfected with overexpression of LINC02147 vector),si-SFRP1 group(60 mg·L^(-1)arecoline treatment,transfected with overexpression of LINC02147 vector,transfected with SFRP1 inhibitor vector).Real-time fluorescence quantitative polymerase chain reaction was used to detect the relative expression levels of SFRP1 mRNA in each group of cells;immunofluorescence was used to detect the positive expression of alpha-smooth muscle ac tin(α-SMA)and vimentin in each group of cells;5-ethynyl-2'-deoxyuridine was used to detect the proliferation rate of each group of cells;dichlorodihydrofluorescein diacetate fluorescence probe method was used to detect the content of reactive oxygen species(ROS)in each group of cells.Results The relative expression levels of SERP1 mRNA in model group and oe-LINC02147 group were 1.00±0.08and 3.75±0.59,respectively;the cell proliferation rates of NC group,model group,oe-LINC02147 group and siSFRP1 group were(43.61±6.97)%,(12.98±4.27)%,(38.66±5.12)%and(15.73±5.42)%,respectively;ROS content were(9.42±1.73)%,(36.58±6.16)%,(12.89±2.08)%and(33.45±5.76)%,respectively;α-SMA positive expression were 1.00±0.13,5.02±1.46,2.36±0.48 and 4.37±0.97,respectively;the positive expressions of vimentin were 1.00±0.15,4.63±1.51,1.98±0.85 and 3.92±1.36,respectively.The above indexes in the model group were compared with the NC group,the above indexes in the oe-LINC02147 group were compared with the model group,and the above indexes in the si-SFRP1 group were compared with the oeLINC02147 group,and the differences were statistically significant(all P<0.001).Conclusion LINC02147 may inhibit the malig

关 键 词：长链非编码RNA 槟榔碱 分泌型卷曲相关蛋白1 人口腔黏膜成纤维细胞 

分 类 号：R97[医药卫生—药品]