炙红芪对脾气虚大鼠盲肠中短链脂肪酸代谢水平的影响  

Effects of honey-processed Hedysari Radix on short-chain fatty acid metabolism in cecum of rats with spleen qi deficiency

作　　者：李越峰 宋沁洁 王毛毛 王哲 马定财 张育贵 刘婷 辛二旦 孙宇靖 严兴科 LI Yue-feng;SONG Qin-jie;WANG Mao-mao;WANG Zhe;MA Ding-cai;ZHANG Yu-gul;LIU Ting;XIN Er-dan;SUN Yu-jing;YAN Xing-ke(School of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;Scientific Research and Experimental Center,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]甘肃中医药大学科研实验中心,甘肃兰州730000

出　　处：《中国临床药理学杂志》2025年第3期397-402,共6页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(82160750);甘肃省教育厅产业支撑计划基金资助项目(2021CYZC-21);甘肃省科技计划(创新基地与人才计划)基础研究创新群体基金资助项目(21JR7RA569);甘肃省重点人才项目;甘肃省联合科研基金资助项目;高等学校产业支撑计划基金资助项目(2024CYZC-41)。

摘　　要：目的探究炙红芪对脾气虚大鼠盲肠中短链脂肪酸(SCFAs)代谢水平的影响。方法将48只SPF级SD雄性大鼠随机分为空白组、模型组、对照组和实验组,每组8只。空白组正常饲养、自由饮水进食,连续15 d,其余组用苦寒泻下、劳倦过度及饥饱失常三因素复合造模法构建脾气虚模型大鼠,连续造模15 d。模型复制成功后,对照组灌胃给予62.5 mg·kg^(-1)双歧杆菌乳杆菌三联活菌片混悬液,实验组灌胃给予炙红芪水提物12.6 g·kg^(-1),空白组灌胃给予等剂量蒸馏水,模型组持续进行造模,连续15 d。用气相色谱质谱联用(GC-MS)法测定各组大鼠盲肠内容物中SCFAs含量。结果空白组大鼠盲肠内容物中乙酸、异丁酸、丁酸、异戊酸、己酸含量分别为(3706.24±401.84)、(99.34±39.11)、(2567.95±529.44)、(86.50±54.11)和(393.14±103.33)μg·g^(-1)。模型组与空白组相比,脾气虚大鼠盲肠内容物中乙酸、异丁酸、丁酸、异戊酸、己酸的含量均显著降低,分别为(3049.48±590.62)、(67.27±12.99)、(2058.00±417.46)、(43.10±11.12)和(27.98±14.40)μg·g^(-1)(P<0.05,P<0.01)。实验组与模型组相比,大鼠盲肠内容物中异戊酸、己酸含量均显著升高,分别为(90.59±26.03)和(60.05±19.89)μg·g^(-1)(均P<0.01)。结论炙红芪可以通过改善脾气虚大鼠盲肠内容物代谢水平,调节盲肠内容物中SCFAs代谢水平,保护肠黏膜屏障、减少肠道炎症反应,从而发挥健脾补气的作用。Objective To explore the effect of honey-processed Hedysari Radix on the metabolism of short-chain fatty acids(SCFAs)in the cecum of rats with spleen qi deficiency.Methods A total of 48SPF male SD rats were randomly divided into blank group,model group,control group and experimental group,with 8 rats in each group.The blank group was fed normally,drank water and ate freely for 15 days,and the other groups used the three-factor composite modeling method of bitter cold diarrhea,overwork and hunger and satiety to construct a spleen qi deficiency model rats for 15 days.After the successful replication of the model,the rats in the control group were given 62.5 mg·kg^(-1)bifidobacterium Lactobacillus triple viable tablets suspension,the experimental group was given 12.6 g·kg^(-1)honey-processed Hedysari Radix decoction,and the blank group was given the same dose of distilled water,and the model group continued to model for 15 days.Gas chromatography-mass spectrometry(GC-MS)was used to determine the contents of SCFAs in the cecal contents of rats in each group.Results The contents of acetic acid,isobutyric acid,butyric acid,isovaleric acid and caproic acid in the cecal contents of rats in the blank group were(3706.24±401.84),(99.34±39.11),(2567.95±529.44),(86.50±54.11)and(393.14±103.33)μg·g^(-1),respectively.Compared with the blank group,the contents of acetic acid,isobutyric acid,butyric acid,isovaleric acid and caproic acid in the cecum contents of spleen-qi deficiency rats in the model group were significantly reduced,which were(3049.48±590.62),(67.27±12.99),(2058.00±417.46),(43.10±11.12)and(27.98±14.40)μg·g^(-1),respectively(P<0.05,P<0.01).Compared with the model group,the contents of isovaleric acid and caproic acid in the cecum contents of rats in the experimental group were significantly increased,which were(90.59±26.03)and(60.05±19.89)μg·g^(-1),respectively(all P<0.01).Conclusion Honey-processed Hedysari Radix can play a role in strengthening the spleen and replenishing qi by improving the me

关 键 词：炙红芪 脾气虚证 气相色谱质谱联用法 短链脂肪酸 肠黏膜屏障 

分 类 号：R28[医药卫生—中药学]