汉黄芩苷上调miR-573表达抑制胰腺癌SW1990细胞增殖、迁移及侵袭的研究  

Research of wogonoside inhibiting the proliferation,migration and invasion of pancreatic cancer SW1990 cells by up-regulating the expression of miR-573

作　　者：王晨曦 陈中建[1] 延学军[1] 刘洪锋[1] 马铎 刘瑶 李永坤[1] WANG Chen-xi;CHEN Zhong-jian;YAN Xue-jun;LIU Hong-feng;MA Duo;LIU Yao;LI Yong-kun(Department of General Surgery,The First Affiliated Hospital of Nanyang Medical College,Nanyang 473000,1Henan Province,China)

机构地区：[1]南阳医学高等专科学校第一附属医院普外科,河南南阳473000

出　　处：《中国临床药理学杂志》2025年第4期482-486,共5页The Chinese Journal of Clinical Pharmacology

基　　金：南阳市科技攻关计划基金资助项目(KJGG150)。

摘　　要：目的探讨汉黄芩苷对胰腺癌SW1990细胞恶性生物学行为的影响,及其作用机制。方法体外培养人胰腺癌细胞SW1990,并随机分为对照组(正常培养)、miR-NC组(转染miR-NC)、miR-573组(转染miR-573 mimics)、anti-miR-NC组(转染anti-miR-NC+20μmol·L^(-1)汉黄芩苷)、anti-miR-573组(转染anti-miR-573+20μmol·L^(-1)汉黄芩苷)和低、中、高剂量实验组(分别给予5、10、20μmol·L^(-1)汉黄芩苷)。用噻唑蓝、平板克隆形成实验、划痕实验和Transwell实验分别检测细胞的增殖、迁移及侵袭能力,用实时荧光定量聚合酶链反应法检测微小RNA-573(miR-573)的表达水平,用蛋白质印迹法检测E-钙黏蛋白(E-cadherin)和N-cadherin蛋白的表达水平。结果高剂量实验组、对照组、miR-NC组、miR-573组、anti-miR-NC组和anti-miR-573组的抑制率分别为(65.37±5.17)%、0、(6.13±0.58)%、(51.94±4.99)%、(67.74±4.38)%和(28.23±2.47)%,划痕愈合率分别为(27.33±2.52)%、(73.47±6.56)%、(74.27±6.86)%、(36.42±3.14)%、(25.91±2.53)%和(61.49±5.11)%,侵袭细胞数分别为(58.05±5.46)、(124.21±10.25)、(126.77±11.89)、(64.74±5.63)、(56.65±4.79)和(103.94±10.68)个,miR-573相对表达水平分别为3.11±0.26、1.00±0.00、1.00±0.00、3.96±0.37、1.00±0.00和0.35±0.03,E-cadherin蛋白相对表达水平分别为0.56±0.04、0.16±0.02、0.15±0.02、0.50±0.04、0.58±0.04和0.28±0.03,N-cadherin蛋白相对表达水平分别为0.24±0.02、0.66±0.04、0.68±0.05、0.31±0.03、0.23±0.02和0.55±0.05。高剂量实验组的上述指标与对照组比较,miR-573组的上述指标与miR-NC组比较,anti-miR-573组的上述指标与anti-miR-NC组比较,在统计学上差异均有统计学意义(均P<0.05)。结论汉黄芩苷可以通过上调miR-573表达,降低N-cadherin表达,升高E-cadherin表达,从而降低胰腺癌细胞的增殖、迁移及侵袭能力。Objective To investigate the effects and mechanism of wogonoside on the malignant biological behavior of pancreatic cancer SW1990 cells.Methods Human pancreatic cancer cells SW1990 were cultured in vitro and randomly divided into control group(normal culture),miR-NC group(transfected with miR-NC),miR-573 group(transfected with miR-573 mimics),anti-miR-NC group(transfected with miR-NC+20μmol·L^(-1)wogonoside),anti-miR-573 group(transfected with anti-miR-573+20μmol·L^(-1)wogonoside)and experimental-L,-M,-H groups(given 5,10 and 20μmol·L^(-1)wogonoside,respectively).The ability of cell proliferation,migration and invasion were detected by methyl thiazolyl tetrazolium assay,plate cloning assay,scratch assay and Transwell assay,respectively;the expression level of microRNA(miR-573)was detected by real-time fluorescence quantitative polymerase chain reaction;Western blot were used to detect the expression levels of E-cadherin and N-cadherin protein.Results The inhibition rates of experimental-H group,control group,miR-NC group,miR-573 group,anti-miR-NC group and anti-miR-573 group were(65.37±5.17)%,0,(6.13±0.58)%,(51.94±4.99)%,(67.74±4.38)%and(28.23±2.47)%,respectively;the scratch healing rates were(27.33±2.52)%,(73.47±6.56)%,(74.27±6.86)%,(36.42±3.14)%,(25.91±2.53)%and(61.49±5.11)%,respectively;the number of invading cells were 58.05±5.46,124.21±10.25,126.77±11.89,64.74±5.63,56.65±4.79 and 103.94±10.68,respectively;the relative expression levels of miR-573 were 3.11±0.26,1.00±0.00,1.00±0.00,3.96±0.37,1.00±0.00 and 0.35±0.03,respectively;the relative expression levels of E-cadherin protein were 0.56±0.04,0.16±0.02,0.15±0.02,0.50±0.04,0.58±0.04 and 0.28±0.03,respectively;the relative expression levels of N-cadherin protein were 0.24±0.02,0.66±0.04,0.68±0.05,0.31±0.03,0.23±0.02 and 0.55±0.05,respectively.The differences of above indexes were statistically significant between the experimental-H group and the control group,between the miR-573 group and the miR-NC group,and between t

关 键 词：汉黄芩苷 胰腺癌 微小RNA-573 细胞增殖 迁移 侵袭 

分 类 号：R28[医药卫生—中药学]