基于转录组技术筛选豁眼鹅眼睑缺损性状的候选基因  

Identification of Candidate Genes for Congenital Eyelid Coloboma Traits in Huoyan Goose by Transcriptome

作　　者：周宁 滕兴怡 李坤[1] 徐亚安 何艳婷 刘鑫蕾 张晶 张名爱[1,2] 王宝维[1,2] 凡文磊 ZHOU Ning;TENG Xingyi;LI Kun;XU Ya′an;HE Yanting;LIU Xinlei;ZHANG Jing;ZHANG Ming′ai;WANG Baowei;FAN Wenlei(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao,Shandong 266109;Institute of Quality Waterfowl,Qingdao Agricultural University,Qingdao,Shandong 266109)

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]青岛农业大学优质水禽研究所,山东青岛266109

出　　处：《中国家禽》2025年第5期25-30,共6页China Poultry

基　　金：国家自然科学基金青年项目(32202668);国家级大学生创新训练计划项目(202310435138);财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-42-14)。

摘　　要：研究旨在通过比较豁眼鹅正常眼和豁眼个体的胚胎期眼睑转录组,筛选豁眼性状的关键候选基因。试验选择18胚龄的豁眼鹅胚胎,分别挑选出3只豁眼和3只正常眼个体,采集发生缺损部位眼睑组织,进行转录组测序和分析。结果显示:豁眼组与正常眼组相比,共有252个差异表达基因,其中上调基因有92个,下调基因160个;结合GO和KEGG数据库对DEGs进行功能注释和通路分析显示,DEGs显著富集到细胞迁移、胞葬作用、神经活性配体—受体相互作用、免疫系统过程等信号通路;进一步结合PPI网络分析等手段,筛选出TACR2、CCL19、DIO1、LRIT3等可能与鹅豁眼性状相关的候选基因。研究表明,豁眼鹅眼睑缺损性状的形成可能与细胞迁移、胞葬等信号通路有关,相关通路中的TACR2、CCL19、DIO1、LRIT3基因可能是眼睑缺损性状的关键基因。The study was aimed to compare the transcriptome of eyelid tissue from Huoyan goose with congenital eyelid colobomas(CEC)or normal eyelids during the embryonic period and to identify the key genes associated with CEC.To collect eyelid tissues for transcriptome,three individuals with CEC eyelid or norm eyelid were selected from embryos of Huoyan geese at 18 d.The results showed that compared with the geese with normal eyelid,there were 252 differentially expressed genes,including 92 up-regulated genes and 160 down-regulated genes.Functional annotation and KEGG pathway analysis of DEGs showed that DEGs were mainly enriched in pathways,such as cell motility,efferocytosis,neuroactive ligand-receptor interaction and immune system processes,etc.By protein-protein interaction(PPI)network analysis,genes such as TACR2,CCL19,DIO1 and LRIT3,were identified as candidate genes.The results suggested that the formation of CEC in Huoyan goose might be related with the the pathways such as cell motility,efferocytosis,and the genes such as TACR2,CCL19,DIO1,LRIT3 that involved in those pathways might be the key gene of CEC formation.

关 键 词：豁眼鹅 转录组 先天性眼睑缺损 分子机制 

分 类 号：S835.2[农业科学—畜牧学]