草地早熟禾白粉病抗性反应中防御酶活性及代谢组学差异  

Differences in Defense Enzyme Activities and Metabolomics in the Resistance Response of Poa pratensis to Powdery Mildew

作　　者：王彦 张玉娟 马福钦 郑晓琳 晋明慧 乃国栋 董文科[1] WANG Yan;ZHANG Yujuan;MA Fuqin;ZHENG Xiaolin;JIN Minghui;NAI Guodong;DONG Wenke(Pratacultural College,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.Center for Grassland Ecosystem Sustainability/Key Laboratory of Forage Germplasm Innovation and Variety Breeding of the Ministry of Agriculture and Rural Affairs,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心/农业农村部饲草种质创新与新品种选育重点实验室,甘肃兰州730070

出　　处：《中国草地学报》2025年第4期12-24,共13页Chinese Journal of Grassland

基　　金：甘肃农业大学公招博士科研启动经费项目(GAUKYQD-2020-31)。

摘　　要：为明确不同抗病性草地早熟禾品种对白粉病菌侵染的防御反应,采用叶面喷施法对草地早熟禾抗病品种‘黑杰克’和感病品种‘超级格莱德’接种禾布氏白粉菌,并取发病部位叶片组织测定相关防御酶活性及代谢产物。结果表明,抗病品种‘黑杰克’接种白粉病菌后各防御酶活性均较第0 d显著提高(P<0.05);感病品种‘超级格莱德’接种白粉病菌后CAT活性始终保持下降趋势,其他防御酶活性均表现为小幅度上升后快速下降。广泛非靶向代谢组学分析发现,与不接种对照相比,抗病品种‘黑杰克’接菌后410种代谢物差异显著,其中195种显著上调,215种显著下调,抗病相关差异代谢物主要为绿原酸、肉桂醛、香豆素及其衍生物等,参与的代谢途径主要为苯丙烷代谢途径;感病品种‘超级格莱德’接菌后547种代谢物差异显著,其中381种显著上调,166种显著下调,抗病相关差异代谢物主要为二羟基香豆素、绿原酸、7-乙氧基香豆素等,主要参与苯丙烷代谢途径等;根据以上结果,推测香豆素类化合物与苯丙烷代谢途径是草地早熟禾响应白粉病菌侵染的关键代谢物与代谢途径。综上,受到白粉病菌侵染时,草地早熟禾抗病品种防御酶活性较感病品种更高,其相关差异代谢物参与苯丙烷代谢途径。In order to clarify the defense responses of different resistant Poa pratensis cultivars to powdery mildew infection,the resistant cultivar‘Black Jack’and the susceptible cultivar‘Ever Glade’were inoculated with Blumeria graminis by foliar spraying,leaf tissues from symptomatic areas were subsequently sampled for analyses of related defense enzyme activities and metabolic product profiles.The results showed that after the resistant cultivar‘Black Jack’was inoculated with powdery mildew,the activities of related defense enzymes were significantly higher than those on the 0th day(P<0.05).After inoculation with powdery mildew,the CAT activity of the susceptible cultivar‘Ever Glade’always maintained a downward trend,while other defense enzymes showed a small increase followed by a rapid decline.Extensive non-targeted metabolomic analysis revealed that 410 metabolites were significantly different between the resistant cultivar‘Black Jack’and the control,of which 195 were significantly up-regulated and 215 were significantly down-regulated,chlorogenic acid,cinnamaldehyde,coumarin and their derivatives were the main differential metabolites related to disease resistance,and phenylpropanoid pathway was the main metabolic pathway.After the susceptible cultivar‘Ever Glade’was inoculated with the pathogen,547 metabolites were significantly different,of which 381 were significantly up-regulated and 166 were significantly down-regulated,the differential metabolites related to disease resistance were mainly dihydroxycoumarin,chlorogenic acid,and 7-ethoxycoumarin,which were mainly involved in phenylpropanoid metabolic pathway.Based on the above results,it is speculated that coumarin compounds and phenylpropane metabolic pathways are the key metabolites and metabolic pathways of Poa pratensis in response to powdery mildew infection.In conclusion,the activity of defense enzymes against powdery mildew infection in diseaseresistant cultivars of Poa pratensis was higher than that in susceptible cultivars,and

关 键 词：草地早熟禾 白粉病 防御酶 代谢组学 抗病性 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]