兽用穿琥宁注射液在犬体内的药代动力学研究  

Study on Pharmacokinetics of Chuanhuning Injection for Veterinary Use in Dogs

作　　者：余佳欣 吴禹熹 章文丽 马春花 苏志鹏 董可 YU Jia-xin;WU Yu-xi;ZHANG Wen-li;MA Chun-hua;SU Zhi-peng;DONG Ke(Sichuan Yugiang Herbal Biotechnology Co.,Ltd.,Chengdu 611100,China;Sichuan Dekang Research and Innovation Technology Co.,Ltd.,Chengdu 610000,China)

机构地区：[1]四川育强本草生物技术有限公司,成都611100 [2]四川德康攻关创新科技有限公司,成都610000

出　　处：《中国兽药杂志》2025年第4期40-44,共5页Chinese Journal of Veterinary Drug

摘　　要：为考察兽用穿琥宁注射液经颈部背侧皮下注射在靶动物比格犬体内的药动学行为。本试验取雌雄各半的比格犬8只,按照5 mg/kgBW剂量经比格犬颈部背侧皮下注射兽用穿琥宁注射液1次,分别在给药前及给药后0.083、0.25、0.50、1、2、4、6、8、12、16、24、36、48、60、96 h采血2 mL,离心取上层血浆,采用HPLC加内标法,检测血浆中脱水穿心莲内酯琥珀酸半酯(DAS)含量,应用药动学软件WinNonlin6.4进行相关药动学参数的计算。结果显示:比格犬血浆中DAS的C_(max)为21.51μg/mL,T_(max)为2.25 h,T_(1/2)为21.51 h,AUC_(0-∞)为29480.36 min·μg/mL。结果表明:本试验分析方法准确、重现性好,比格犬皮下注射穿琥宁注射剂后,在体内达峰时间较快,半衰期较长,为临床制定合理的给药方案提供试验依据。To explore the pharmacokinetic behavior of chaunhuning injection when administered subcutaneously to the dorsal neck of the target animal,the beagle dog.In this experiment,8 male and female beagle dogs were given a subcutaneous injection at the back of the neck at a dose of 5 mg/kgBW.2 mL of blood was collected before and after administration at 0.083、0.25、0.50、1、2、4、6、8、12、16、24、36、48、60 and 96 h,respectively.The content of DAS in plasma was determined by the HPLC and internal standard method.The pharmacokinetic parameters were calculated using WinNonlin6.4.The results indicated that the C_(max) of DAS in beagle plasma was 21.51μg/mL,T_(max)was 2.25 h,T_(1/2)was 21.51 h,and AUC_(0-∞)was 29480.36 min·μg/mL.The results demonstrated that the analytical method was accurate and reproducible.After subcutaneous injection of chuanhuning injection in beagle dogs,the peak time in vivo was quicker and the half-life was longer,providing an experimental basis for the clinical formulation of reasonable drug administration.

关 键 词：穿琥宁注射液 犬 药代动力学 

分 类 号：S859.79[农业科学—临床兽医学]