羟基红花黄色素A通过神经营养因子-3对大鼠胫骨骨骺损伤的作用  

Effects of hydroxylsafflower yellow A on epiphyseal injury of tibial bone in rats through neurotrophin-3

作　　者：任晓春[1] 李文斌[1] 赵致忠 吕嘉[1] 黄永波[1] REN Xiao-chun;LI Wen-bin;ZHAO Zhi-zhong;LU Jia;HUANG Yong-bo(Department of Orthopedics,The Second Hospital of Shanxi Medical University,Taiyuan 030000,Shanxi Province,China)

机构地区：[1]山西医科大学第二医院骨科,山西太原030000

出　　处：《中国临床药理学杂志》2025年第5期696-700,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究羟基红花黄色素A(HSYA)通过神经营养因子-3(NT-3)对大鼠胫骨骨骺损伤的影响机制。方法将50只雄性SD大鼠随机分为对照组、模型组、HSYA组、NC组和sh-NT-3组,每组10只。除对照组外,其余4组均用高速牙钻建立胫骨骨骺损伤模型。HSYA组灌胃给予10 mg·kg^(-1) HSYA,qd,连续6周;NC组和sh-NT-3组分别在HSYA组的基础上尾静脉注射1 mg·kg^(-1) sh-NC和sh-NT-3,每2天1次,连续6周。对照组和模型组给予等量的0.9%NaCl灌胃和尾静脉注射。用实时定量反转录聚合酶链反应检测NT-3 mRNA相对表达水平,用蛋白质印迹法检测骨修复标志物和Notch通路关键基因Notch受体1(Notch1)信号通路相关蛋白的相对表达水平。结果对照组、模型组、HSYA组的NT-3 mRNA相对表达水平分别为1.00±0.17、3.31±0.59和5.16±0.84;对照组、模型组、HSYA组、NC组和sh-NT-3组的Runt相关转录因子2蛋白相对表达水平分别为1.00±0.17、3.67±0.63、7.36±1.35、7.31±1.42和5.74±1.32,成骨细胞特异性转录因子蛋白相对表达水平分别为1.00±0.18、5.76±1.01、8.49±1.42、8.56±1.54和6.48±1.16,Notch1蛋白相对表达水平分别为1.00±0.18、1.44±0.23、1.79±0.34、1.83±0.35和1.52±0.27。模型组的上述指标与对照组比较,HSYA组的上述指标与模型组比较,sh-NT-3组的上述指标与NC组比较,在统计学上差异均有统计学意义(均P<0.05)。结论HSYA通过促进NT-3表达,激活Notch1信号通路改善大鼠胫骨骨骺损伤。Objective To study the effect of hydroxylsafflower yellow A(HSYA)on epiphyseal injury of tibial in rats through neurotrophic factor-3(NT-3).Methods Fifty male SD rats were randomly divided into control group,model group,HSYA group,NC group and sh-NT-3group,with 10 rats in each group.Except the control group,the injury model of tibial epiphyseal was established in the other 4 groups by high speed dental abrasive drill.The HSYA group was given 10 mg·kg^(-1)HSYA by intragastric administration once a day for 6 weeks.NC group and sh-NT-3 group were respectively injected with 1 mg·kg^(-1)sh-NC and sh-NT-3 through the tail vein,once every two days for 6 weeks.The control group and model group were given the same amount of normal saline intragastric and caudal intravenous injection.Quantitative realtime polymerase chain reaction was used to detect the expression level ofNT-3 mRNA.The expression levels of bone repair markers and Notch receptor 1(Notch1)signaling pathway related proteins were detected by Western blot.Results The mRNA levels ofNT-3 in control group,model group and HSYA group were 1.00±0.17,3.31±0.59and 5.16±0.84,respectively.The relative expression of runt related transcription factor 2 protein in control group,model group,HSYA group,NC group and sh-NT-3 group were 1.00±0.17,3.67±0.63,7.36±1.35,7.31±1.42,5.74±1.32,respectively;the relative expressions of osteoblast-specific transcription factor protein were 1.00±0.18,5.76±1.01,8.49±1.42,8.56±1.54,6.48±1.16,respectively;the relative expressions of Notch1 protein were 1.00±0.18,1.44±0.23,1.79±0.34,1.83±0.35,1.52±0.27,respectively.The above indexes in the model group were compared with the control group,the HSYA group was compared with the model group,and the sh-NT-3 group was compared with the NC group,and the above indexes were statistically significant(allP<0.05).Conclusion HSYA improves tibial epiphyseal injury in rats by promoting NT-3 expression and activating Notch1 signaling pathway.

关 键 词：羟基红花黄色素A 神经营养因子-3 骨骺损伤 Notch1信号通路 胫骨 

分 类 号：R28[医药卫生—中药学]