高良姜素改善血管性痴呆小鼠抑郁样行为的研究  

Research of galangin improving depression-like behavior in vascular dementia mice

作　　者：贺佳 孔东燕[1] HE Jia;KONG Dong-yan(Psychological Clinic,Quanzhou First Hospital Quanzhou,Quanzhou 362000,Fujian Province,China)

机构地区：[1]泉州市第一医院心理门诊,福建泉州362000

出　　处：《中国临床药理学杂志》2025年第5期701-705,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究高良姜素通过微小RNA-134(miRNA-134)改善血管性痴呆(VD)小鼠抑郁样行为的作用及其机制。方法将70只C57BL/6J小鼠随机分为假手术组(0.9%NaCl)、模型组(0.9%NaCl)、低剂量组(25 mg·kg^(-1)高良姜素)、中剂量组(50 mg·kg^(-1)高良姜素)、高剂量组(100 mg·kg^(-1)高良姜素)、mimic NC组(100 mg·kg^(-1)高良姜素和mimic NC 15 nmol)、miRNA-134 mimic组(100 mg·kg^(-1)高良姜素和miRNA-134 mimic 15 nmol)。用悬尾实验和强迫游泳实验检测抑郁程度,用酶联免疫吸附试验法检测皮质酮水平,用实时荧光定量聚合酶链反应检测miRNA-134水平,用脱氧核糖核苷酸末端转移酶介导的缺口末端标记实验检测脑组织细胞凋亡率,用蛋白质印迹法检测突触可塑性相关蛋白水平。结果假手术组、模型组和高剂量组的悬尾不动时间分别是(105.32±15.22)、(185.32±24.62)和(112.65±15.08)s,游泳不动时间分别是(86.52±13.25)、(152.32±22.22)和(96.22±16.35)s,皮质酮水平分别是(32.65±5.02)、(86.62±14.32)和(48.52±5.55)ng·L^(-1),miRNA-134相对表达水平分别是1.00±0.12、2.32±0.35和1.21±0.18;假手术组、模型组、高剂量组、mimic NC组和miRNA-134 mimic组细胞凋亡率分别是(5.32±0.62)%、(62.33±8.65)%、(14.32±1.78)%、(15.85±2.22)%和(43.21±7.05)%,突触素(SYP)蛋白相对表达水平分别是1.00±0.18、0.32±0.05、0.88±0.12、0.82±0.11和0.30±0.05,突触相关蛋白95(PSD-95)蛋白相对表达水平分别是1.00±0.14、0.61±0.07、0.72±0.09、0.70±0.08和0.45±0.06,模型组的上述指标与假手术组比较,高剂量组的上述指标与模型组比较,miRNA-134 mimic组的上述指标与mimic NC组比较,在统计学上差异均有统计学意义(均P<0.05)。结论高良姜素可能通过抑制miRNA-134表达,改善神经突触可塑性,抑制细胞凋亡,进而改善VD小鼠抑郁样行为。Objective To study the effect and mechanism of galangin in improving depression-like behavior in vascular dementia(VD)mice through microRNA-134(miRNA-134).Methods Seventy C57BL/6J mice were randomly divided into sham group(0.9%NaCl),model group(0.9%NaCl),low-dose group(25 mg·kg^(-1)galangin),medium-dose group(50 mg·kg^(-1)galangin),high-dose group(100 mg·kg^(-1)galangin),and mimic NC group(100 mg·kg^(-1)galangin and mimic NC 15 nmol),miRNA-134 mimic group(100 mg·kg^(-1)galangin and miRNA-134 mimic 15 nmol).The degree of depression was detected by tail suspension test and forced swimming test;the level of corticosterone was detected by enzyme-linked immunosorbent assay;the level ofmiRNA-134 was detected by quantitative real time polymerase chain reaction;the apoptosis rate of brain tissue cells was detected by TdT-mediated dUTP nick-end labeling;and the level of synaptic plasticity related protein was detected by Western blot.Results The suspension time of the sham group,the model group and the high-dose group were(105.32±15.22),(185.32±24.62)and(112.65±15.08)s,respectively;the swimming time were(86.52±13.25),(152.32±22.22)and(96.22±16.35)s respectively;the levels of corticosterone were(32.65±5.02),(86.62±14.32)and(48.52±5.55)ng·L^(-1),respectively;the levels ofmiRNA-134 were 1.00±0.12,2.32±0.35 and 1.21±0.18,respectively.The apoptosis rates of the sham group,model group,high group,mimic NC group and miRNA-134mimic group were(5.32±0.62)%,(62.33±8.65)%,(14.32±1.78)%,(15.85±2.22)%and(43.21±7.05)%,respectively;synaptophysin protein levels were 1.00±0.18,0.32±0.05,0.88±0.12,0.82±0.11 and 0.30±0.05,respectively;the postsynaptic density protein-95 protein levels were 1.00±0.14,0.61±0.07,0.72±0.09,0.70±0.08 and 0.45±0.06,respectively.The above indexes in the model group were compared with those in the sham group,those in the high-dose group were compared with those in the model group,and those in the miRNA-134 mimic group were compared with those in the mimic NC group,and the differences

关 键 词：高良姜素 神经突触 血管性痴呆 抑郁 微小RNA-134 

分 类 号：R28[医药卫生—中药学]