柴达木盆地不同地区枸杞根际土壤丛枝菌根真菌群落组成  

Composition of Arbuscular Mycorrhizal Fungal Community in the Rhizosphere Soil of Lycium barbarum in Different Regions of the Qaidam Basin

作　　者：李淑慧 郭寰 季生连 李建领 樊光辉[1] 段国珍 Li Shuhui;Guo Huan;Ji Shenglian;Li Jianling;Fan Guanghui;Duan Guozhen(Qinghai University,Xining 810016,P.R.China)

机构地区：[1]青海大学,西宁810016

出　　处：《东北林业大学学报》2025年第5期106-115,共10页Journal of Northeast Forestry University

基　　金：青海省重大科技专项项目(2023-NK-A4)。

摘　　要：为提高丛枝菌根真菌(AMF)在柴达木盆地生态修复、增强植物抗性中的应用基础,以青海省柴达木盆地9个地区(都兰县翔宇有限公司(XY)、都兰县一大队(NY)、都兰县四大队(NSI)、都兰县五大队(NW)、都兰县三大队(NS)、都兰县二大队(NE)、格尔木市大格勒乡(KP)、格尔木市郭勒木德镇(GEM)、德令哈市怀头他拉镇(WSJ))的枸杞根际土壤为研究对象,采用Illumina高通量测序对根际土壤丛枝菌根真菌的多样性和群落结构进行测定分析,通过冗余分析探明根际土壤理化性质、地理距离对丛枝菌根真菌多样性及相对丰度的影响。结果表明:对根际土壤中丛枝菌根真菌群落结构组成进行比较发现,9个样地共有的特征数量为97个,可划分为4目8科9属。其中,球囊霉属(Glomus)是特征数量最多的属。韦恩图分析可知,XY样地特征数量最多,KP样地特征数量最少;α多样性显示,NSI样地群落结构多样性和丰富度均显著高于其余地区,而GEM样地群落结构多样性和丰富度为最低;通过土壤理化性质与地理距离分析显示,土壤pH、全磷、碱解氮和速效钾质量分数是影响丛枝菌根真菌群落结构的关键因子;不同样地枸杞根际土壤丛枝菌根真菌群落相似性存在明显的地理衰减格局。总体而言,不同样地的枸杞根际土壤中丛枝菌根真菌群落结构具有一定差异。To improve the application foundation of arbuscular mycorrhizal fungi(AMF)in ecological restoration and enhancing plant resistance in the Qaidam Basin,the rhizosphere soil of Lycium barbarum from nine regions in the Qaidam Basin of Qinghai Province[Xiangyu Co.,Ltd.in Dulan County(XY),the 1st Brigade in Dulan County(NY),the 4th Brigade in Dulan County(NSI),the 5th Brigade in Dulan County(NW),the 3rd Brigade in Dulan County(NS),the 2nd Brigade in Dulan County(NE),Dagle Township in Golmud City(KP),Guolemude Town in Golmud City(GEM),and Huaitoutala Town in Delingha City(WSJ)]was selected as the research object.Illumina high-throughput sequencing was used to measure and analyze the diversity and community structure of arbuscular mycorrhizal fungi in the rhizosphere soil.Redundancy analysis was employed to explore the effects of the physical and chemical properties of the rhizosphere soil and geographical distance on the diversity and relative abundance of arbuscular mycorrhizal fungi.The results showed that when comparing the community structure composition of arbuscular mycorrhizal fungi in the rhizosphere soil,there were 97 common features in the nine sample plots,which could be classified into 4 orders,8 families,and 9 genera.Among them,Glomus was the genus with the largest number of features.According to the Venn diagram analysis,the XY sample plot had the largest number of features,while the KP sample plot had the smallest number.Theα-diversity analysis indicated that the community structure diversity and richness in the NSI sample plot were significantly higher than those in the other regions,while the GEM sample plot had the lowest community structure diversity and richness.Analysis of the physical and chemical properties of the soil and geographical distance revealed that soil pH,total phosphorus,available nitrogen,and available potassium contents were the key factors influencing the community structure of arbuscular mycorrhizal fungi.There was an obvious geographical attenuation pattern in the similarity of

关 键 词：柴达木盆地 枸杞 根际土壤丛枝菌根真菌 群落结构 

分 类 号：S154.3[农业科学—土壤学]