防治冷杉梢斑螟的球孢白僵菌液体发酵优化  

Optimization of Liquid Fermentation of Beauveria bassiana for the Control of Dioryctria abietella

作　　者：邱云腾 陈汝婷 王美玲[1] 程金藏 宇佳[1] 迟德富[1] Qiu Yunteng;Chen Ruting;Wang Meiling;Cheng Jincang;Yu Jia;Chi Defu(Northeast Forest University,Harbin 150040,P.R.China)

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2025年第5期144-152,共9页Journal of Northeast Forestry University

基　　金：“十四五”国家重点研发计划项目(2022YFD1401000);黑龙江省重点研发计划项目(2023ZX02B05)。

摘　　要：为研制出对冷杉梢斑螟(Dioryctria abietella)具有高致病性的生物农药,选用对其幼虫具有高致病性的球孢白僵菌(Beauveria bassiana)进行前期的液体发酵技术研究,便于为后续的固体发酵规模化生产奠定基础。采用单因素方差分析的方法,将原培养基中的碳源和氮源成分进行替换,并添加了无机盐成分,获得较高的菌丝生长量。结果表明:适宜球孢白僵菌CFCC81428菌株发酵的最佳碳源是可溶性淀粉,最佳氮源是酵母提取物,最佳无机盐是无水氯化钙。采用响应面分析方法对发酵条件(发酵温度、时间、接种量、培养基初始pH)进行优化,发现菌株CFCC81428在初始pH为7.412、接种体积分数为2.933%、培养温度为28℃、培养4 d时的发酵效果最好。按照此方案进行试验,菌丝生长量达到14.31 g/L,与模型预测值14.172 g/L较为吻合,证明此次优化方案可行。液体发酵过程优化之后的球孢白僵菌菌丝生长量相比于优化前有了明显提高,可为后续固体发酵提供更多原料。In order to develop a biopesticide with high pathogenicity to Dioryctria abietella,Beauveria bassiana,which has high pathogenicity to its larvae,was selected for the preliminary research on liquid fermentation technology,so as to lay a foundation for the subsequent large-scale production of solid fermentation.By using the method of one-way analysis of variance,the carbon source and nitrogen source components in the original medium were replaced,and inorganic salt components were added to obtain a higher mycelial growth amount.The results showed that the optimal carbon source for the fermentation of B.bassiana strain CFCC81428 was soluble starch,the optimal nitrogen source was yeast extract,and the optimal inorganic salt was anhydrous calcium chloride.The response surface analysis method was used to optimize the fermentation conditions(fermentation temperature,time,inoculation amount,initial pH of the medium).It was found that the fermentation effect of strain CFCC81428 was the best when the initial pH was 7.412,the inoculation volume fraction was 2.933%,the culture temperature was 28℃,and the culture time was 4 days.According to this scheme,the mycelial growth amount reached 14.31 g/L,which was in good agreement with the predicted value of the model(14.172 g/L),proving that the optimization scheme was feasible.The mycelial growth amount of B.bassiana after the optimization of the liquid fermentation process was significantly higher than that before the optimization,which can provide more raw materials for the subsequent solid fermentation.

关 键 词：球孢白僵菌 液体发酵 响应面分析 

分 类 号：S767.32[农业科学—森林保护学]