改良Simpson法与二维斑点追踪超声心动图评估大鼠心肌缺血再灌注损伤的对比研究  

Comparative study of the modified Simpson’s method and two-dimensional speckle-tracking echocardiography for assessing myocardial ischemia-reperfusion injury in rats

作　　者：庄太红 何文梅 蔡能 史艺莹 黄泽健[2] 王芳[3] 秦将均[4] 刘宇杰 ZHUANG Taihong;HE Wenmei;CAI Neng;SHI Yiying;HUANG Zejian;WANG Fang;QIN Jiangjun;LIU Yujie(Department of Ultrasound,the First Affiliated Hospital of Hainan Medical University,Haikou 570000,China;Department of Functional Examination,Sanya Central Hospital,Hainan Third People’s Hospital,Sanya 572000,China;Department of Cardiovascular Medicine,Sanya Central Hospital,Hainan Third People’s Hospital,Sanya 572000,China;Department of Radiology,Sanya Central Hospital,Hainan Third People’s Hospital,Sanya 572000,China;the First School of Clinical Medicine,Hainan Medical University,Haikou 571199,China)

机构地区：[1]海南医科大学第一附属医院超声科,海南海口570000 [2]三亚中心医院海南省第三人民医院功能科,海南三亚572000 [3]三亚中心医院海南省第三人民医院心内科,海南三亚572000 [4]三亚中心医院海南省第三人民医院放射科,海南三亚572000 [5]海南医科大学第一临床医学院,海南海口571199

出　　处：《临床超声医学杂志》2025年第4期283-289,共7页Journal of Clinical Ultrasound in Medicine

基　　金：国家自然科学基金项目(82060319);海南省自然科学基金项目(823RC613);海南省研究生创新科研课题(Qhys2023-440)。

摘　　要：目的应用降维机器学习算法评估并比较改良Simpson法与二维斑点追踪超声心动图(2D-STE)评估大鼠心肌缺血再灌注损伤(I/R)的可靠性。方法将36只6~8周龄雄性SD大鼠随机分为I/R组15只、铁死亡抑制剂(Fer-1)+I/R组11只、假手术组10只,并进行对应处理。再灌注后24 h应用改良Simpson法测量大鼠左心室射血分数(LVEF)、左心室舒张末期容积、左心室收缩末期容积及每搏输出量;2D-STE测量左心室乳头肌水平的整体环向收缩期峰值应变(GCSsys)及其中3个节段(前壁、前间隔、后间隔)局部环向应变(RCS);TTC染色法计算大鼠心肌梗死面积占比,比较各组上述参数的差异。采用最小绝对收缩和选择算子(Lasso)算法绘制交叉验证曲线图、轨迹图、瀑布图筛选评估大鼠心肌I/R后左心室收缩功能的优秀参数。结果实验过程中I/R组大鼠死亡5只,Fer-1+I/R组死亡1只,各组最终纳入统计分析的大鼠均为10只。I/R组、Fer-1+I/R组GCSsys和前壁、前间隔、后间隔RCS均低于假手术组,Fer-1+I/R组LVEF、GCSsys和前壁、前间隔、后间隔RCS均高于I/R组,差异均有统计学意义(均P<0.05)。I/R组、Fer-1+I/R组、假手术组心肌梗死面积占比分别为(40.57±7.93)%、(22.55±2.50)%、0,各组两两比较差异均有统计学意义(均P<0.05)。Lasso回归结果显示,2D-STE参数在评估大鼠心肌I/R后左心室收缩功能方面的表现优于改良Simpson参数,且前壁RCS表现最优。结论与改良Simpson法比较,2D-STE能更有效地评估Fer-1对大鼠心肌I/R的保护作用;且前壁RCS是较为敏感的左心室收缩功能监测参数。Objective To apply a dimensionality reduction machine learning algorithm to evaluate and compare the reliability of the modified Simpson’s method and two-dimensional speckle-tracking echocardiography(2D-STE)in assessing myocardial ischemia-reperfusion injury(I/R)in rats.Methods A total of 36 male Sprague-Dawley(SD)rats with aged 6 to 8 weeks was randomly divided into three groups:the I/R group(n=15),the ferroptosis inhibitor(Fer-1)+I/R group(n=11),and the sham operation group(n=10).At 24 h after reperfusion,left ventricular ejection fraction(LVEF),end-diastolic volume(LVEDV),end-systolic volume(LVESV),and stroke volume(SV)were measured by the modified Simpson’s method.2D-STE was used to measure global circumferential strain during systole(GCSsys)at the papillary muscle level and regional circumferential strain(RCS)in three segments(anterior wall,anterior septum,and posterior septum).The percentage of myocardial infarction area was determined by TTC staining.The differences of the above parameters were compared among the groups.Cross-validation curves,coefficient path plots,and waterfall plots were drawn by least absolute shrinkage and selection operator(Lasso)regression to identify optimal parameters for evaluating myocardial I/R injury in rats.Results During the experiment,5 rats died in the I/R group and 1 rat died in the Fer-1+I/R group.Ultimately,10 rats from each group were included in the statistical analysis.GCSsys and RCS of anterior wall,anterior septum and posterior septum in I/R group and Fer-1+I/R group were lower than those of sham operation group.LVEF,GCSsys and RCS of anterior wall,anterior septum and posterior septum in Fer-1+I/R group were higher than those of I/R group,with statistical significances(all P<0.05).The proportion of myocardial infarction area in the I/R group,Fer-1+I/R group and sham operation group were(40.57±7.93)%,(22.55±2.50)%and 0,respectively.There were significant differences in pairwise(all P<0.05).Lasso regression indicated that 2D-STE parameters outperformed those of

关 键 词：超声心动描记术 斑点追踪 二维 心肌缺血再灌注损伤 收缩功能 心室 左 铁死亡抑制剂 大鼠 

分 类 号：R540.45[医药卫生—心血管疾病]