天麻与益生菌复配对大鼠消化功能的促进作用  

Improving Effect of Co-administration of Gastrodia elata and Probiotics on the Digestive Function in Rats

作　　者：吴机伶 郑泽江 金国策 孙莲 左一帆 尉小琴 李啸 谈亚丽 黄蓉 陈剑锋[1] 邹坤[1] WU Jiling;ZHENG Zejiang;JIN Guoce;SUN Lian;ZUO Yifan;YU Xiaoqin;LI Xiao;TAN Yali;HUANG Rong;CHEN Jianfeng;ZOU Kun(Hubei Key Laboratory of Natural Products Research and Development,China Three Gorges University,Yichang 443002,China;College of Medicine and Health Sciences,China Three Gorges University,Yichang 443002,China;Angel Biotechnology Co.,Ltd.,Yichang 443003,China)

机构地区：[1]三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌443002 [2]三峡大学健康医学院,湖北宜昌443002 [3]安琪生物科技有限公司,湖北宜昌443003

出　　处：《食品与生物技术学报》2025年第1期107-114,共8页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金青年基金项目(82204237);湖北省自然科学基金项目(2022CFB827)。

摘　　要：【目的】探讨天麻与益生菌复配对大鼠消化功能的影响及其对肠道菌群的作用。【方法】选用SD大鼠,首先探究天麻提取物对大鼠消化功能的影响,测定提取物中总多酚和总多糖的质量分数,并将提取物与益生菌复配,灌胃30 d后,测定大鼠的体质量、摄食量、食物利用率、胃蛋白酶活性、胃泌素(GAS)和生长抑素(SS)的质量浓度等,并用16S rRNA检测肠道菌群结构。【结果】复配组大鼠的体质量、摄食量、胃蛋白酶活性、胃蛋白酶排出量、GAS质量浓度相对于空白组均显著增加(P<0.05),SS质量浓度显著降低(P<0.05),且天麻与益生菌复配能调整大鼠肠道菌群结构,增加Lactobacillaceae、Ruminococcaceae、Lachnospiraceae等有益菌的相对丰度。【结论】天麻与益生菌复配对促进大鼠消化功能有积极作用,具有一定的开发价值。[Objective]To investigate the effects of co-administration of Gastrodia elata(G.elata)and probiotics on the digestive function and intestinal microbiota in rats.[Method]SD rats were utilized to explore the effects of G.elata extract on the digestive function,and the mass fractions of total phenols and polysaccharides in the extract were determined.The extract was then combined with probiotics for gavage in rats.After 30 d of gavage,the body weight,food intake,food utilization rate,pepsin activity,and mass concentrations of gastrin(GAS)and somatostatin(SS)were measured.The intestinal microbiota structure was analyzed by 16S rRNA sequencing.[Result]The co-administration group showed increases in body weight,food intake,pepsin activity,pepsin excretion,and GAS level compared with the control group(P<0.05),with a decrease in SS level(P<0.05).Additionally,the co-administration of G.elata and probiotics adjusted the intestinal microbiota structure by increasing the relative abundance of beneficial bacteria such as Lactobacillaceae,Ruminococcaceae,and Lachnospiraceae.[Conclusion]The co-administration of G.elata and probiotics positively affects the digestive function in rats and holds the potential for development.

关 键 词：天麻 益生菌 消化功能 胃泌素 生长抑素 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]