猪肺炎支原体脂蛋白LppB介导纤毛黏附功能域的鉴定  

Identification of Functional Motif in Mycoplasma hyopneumoniae LppB Mediating Ciliary Adhesion

作　　者：叶利颖 刘威[2] 闫安 周丹娜[2] 杨克礼[2] 高婷 田永祥[2] 宋淇淇 YE Li-ying;LIU Wei;YAN An;ZHOU Dan-na;YANG Ke-li;GAO Ting;TIAN Yong-xiang;SONG Qi-qi(College of Animal Science and Veterinary Medicine/Tianjin Key Laboratory of Agricultural Animal Breeding and Health Breeding,Tianjin Agricultural University,Tianjin,300384,China;Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Science/Key Laboratory of Prevention and Control Agents for Animal Bacteriosis(Ministry of Agriculture and Rural Affairs)/Hubei Provincial Key laboratory of Animal Pathogenic Microbiology,Wuhan,Hubei,430064,China)

机构地区：[1]天津农学院动物科学与动物医学学院/天津市农业动物繁育与健康养殖重点实验室,天津300392 [2]湖北省农业科学院畜牧兽医研究所/农业农村部畜禽细菌病防治制剂创制重点实验室/畜禽病原微生物学湖北省重点实验室,湖北武汉430064

出　　处：《动物医学进展》2025年第6期1-6,共6页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(32202760);天津市自然科学基金项目(19JCQNJC13700);湖北省农业科技创新中心资助项目(2019-620-000-001-17)。

摘　　要：猪肺炎支原体(Mhp)主要引起地方性肺炎,给全球养猪业造成了巨大的经济损失。LppB是猪肺炎支原体的毒力因子,与纤毛的黏附和呼吸道定植密切相关,然而其具体的功能域还未见报道。利用生物信息学方法,对LppB可能的串联重复区域、disorder功能域、抗原表位和高级结构进行了预测;为了保留预测功能域的完整性,将LppB分成了3个截短片段,依次为ΔLppB 27-247、ΔLppB 248-424和ΔLppB 425-604;通过原核表达系统,表达3个截短片段,获得了表达的rΔLppB 27-247、rΔLppB 248-424和rΔLppB 425-604重组蛋白;借助纤毛黏附试验,测定了截短片段黏附纤毛的能力。结果显示,LppB黏附纤毛的功能域主要位于27-247 aa和425-604 aa区域。研究结果丰富了对猪肺炎支原体致病机制的理解。Mycoplasma hyopneumoniae primarily induces endemic pneumonia,resulting in substantial economic losses to the global swine industry.LppB,a virulence determinant of M.hyopneumoniae,is intricately associated with ciliary adhesion and colonization within the respiratory tract.However,the precise functional motif remains unreported.The present study employed bioinformatics methodologies to predict the possible putative functional motif,tandem repeat regions,epitope,and secondary structure of LppB.In order to maintain the integrity of the function motif,LppB is partitioned into three truncated segments,namelyΔLppB 27-247,ΔLppB 248-424 andΔLppB 425-604.Three truncated proteins were expressed via the prokaryotic expression system,and the expression products of rΔLppB 27-247,rΔLppB 248-424,and rΔLppB 425-604 were obtained in vitro.The adhesion ability of truncated fragments to cilia was quantified using a ciliary adhesion assay.The results demonstrated that the functional motif responsible for LppB adhesion to cilia were primarily localized within the 27-247 aa and 425-604 aa regions.This study significantly enhances our comprehension of the pathogenic mechanism of M.hyopneumoniae.

关 键 词：猪肺炎支原体 LppB 纤毛黏附 功能域 

分 类 号：S852.62[农业科学—基础兽医学] S858.28[农业科学—兽医学]