猫传染性腹膜炎病毒S和3c基因遗传进化分析及其S蛋白免疫原性研究  

Phylogenetic Analysis of Feline Infectious Peritonitis Virus S and 3c Genes and Immunogenicity of Spike Protein

作　　者：张婷[1] 刘童通 李春雨[2] 姚孟 李炜 杨勃 孙子龙[1] 牛胜 张鼎[1] ZHANG Ting;LIU Tong-tong;LI Chun-yu;YAO Meng;LI Wei;YANG Bo;SUN ZI-long;NIU Sheng;ZHANG Ding(College of Veterinary Medicine,Shanxi Agricultural University,Taigu,Shanxi,030801,China;Laboratory Animal Center,Tsinghua University,Beijing,100084,China)

机构地区：[1]山西农业大学动物医学学院,山西太谷030801 [2]清华大学实验动物中心,北京100084

出　　处：《动物医学进展》2025年第6期18-25,共8页Progress In Veterinary Medicine

基　　金：山西省基础研究计划项目(202303021211105);山西省科技创新人才团队专项资助项目(202304051001041,202204051001022)。

摘　　要：猫传染性腹膜炎病毒(FIPV)属于冠状病毒属成员,是引起猫传染性腹膜炎(FIP)发生的病原体。FIPV感染给猫健康造成巨大威胁,阐明FIPV遗传变异特征及主要抗原蛋白免疫原性对FIP防控具有重要意义。扩增4株FIPV山西株S和3c基因,分析FIPV山西株同源性和遗传进化特征。结果显示,4株FIPV山西株间S基因相似性为89.6%~91.9%,与已公布的Ⅰ型猫冠状病毒(FCoV)相似性为85.9%~92.7%。FIPV山西株间3c基因相似性为91.5%~95.3%,与公布的Ⅰ型FCoV参考株相似性为90.9%~95.9%。S、3c基因遗传进化分析显示,4株FIPV山西株均属于Ⅰ型FCoV。将表达、纯化的FIPV S蛋白免疫Balb/c小鼠,间接ELISA检测小鼠血清S蛋白抗体水平,结果显示FIPV S蛋白具有良好的免疫原性,为FIPV感染的诊断与防控提供了重要科学依据。Feline infectious peritonitis virus(FIPV),a member of the genus Coronavirus,is a pathogen that causes feline infectious peritonitis(FIP).FIPV infection poses a great threat to the health of cats.Elucidation of FIPV genetic variation and immunogenicity of major antigen proteins is of great significance for the prevention and control of FIP.The present study amplified S and 3c genes of four FIPV strain isolated from Shanxi province,analyzed the homology and genetic evolution characteristics of FIPV Shanxi strains.The results showed that the S gene similarity among the four FIPV Shanxi strains was 89.6%to 91.9%,and the similarity with the published type I feline coronavirus(FCoV)was 85.9%to 92.7%.The similarity of 3c gene among FIPV Shanxi strains was 91.5%to 95.3%,and the similarity with the published type I FCoV reference strains was 90.9%to 95.9%.The genetic and evolutionary analysis of S and 3c genes showed that all the four FIPV Shanxi strains belonged to the type I FCoV.FIPV S protein was expressed,purified and used to immunize Balb/c mice.Indirect ELISA was used to detect the anti-FIPV-S protein antibody in mice.The results showed that FIPV S protein had good immunogenicity,which provided an important scientific basis for the diagnosis and prevention of FIPV infection.

关 键 词：猫传染性腹膜炎病毒 遗传进化分析 刺突蛋白 3c蛋白 免疫原性 

分 类 号：S852.659.6[农业科学—基础兽医学] S858.293[农业科学—兽医学]