两种不同佐剂猪流感灭活疫苗小鼠皮内免疫效果比较  

Comparison of Intradermal Immune Effect of Swine Influenza Virus Inactivated Vaccines with Two Different Adjuvants

作　　者：阮卓雨 陈超阳 邓碧华[1,2,3] 尹文竹 马芳 卢宇 王海燕[1,2,3] RUAN Zhuo-yu;CHEN Chao-yang;DENG Bi-hua;YIN Wen-zhu;MA Fang;LU Yu;WANG Hai-yan(Institute of Veterinary Immunology&Engineering,National Research Center of Engineering and Technology for Veterinary Biologicals,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu,210014,China;Nanjing Agricultural University,Nanjing,Jiangsu,210095,China;GuoTai(Taizhou)Center of Technology Innovation for Veterinary Biologicals,Taizhou,Jiangsu,225300,China;Sinovet(Jiangsu)Biopharmaceutical Co.,Ltd.,Taizhou,Jiangsu,225300,China)

机构地区：[1]江苏省农业科学院动物免疫工程研究所/国家兽用生物制品工程技术研究中心,江苏南京210014 [2]南京农业大学,江苏南京210095 [3]兽用生物制品(泰州)国泰技术创新中心,江苏泰州225300 [4]华威特(江苏)生物制药有限公司,江苏泰州225300

出　　处：《动物医学进展》2025年第6期83-90,共8页Progress In Veterinary Medicine

基　　金：江苏省自然科学基金面上项目(BK20221431);江苏省农业科技自主创新资金项目(CX(22)2018)。

摘　　要：皮内免疫是一种高效的疫苗免疫途径,亟需安全高效的皮内免疫佐剂。将佐剂Gel02和ISA15分别与猪流感抗原配成疫苗,免疫接种小鼠,分别于免疫后不同时间对小鼠接种部位皮肤反应和抗体水平进行检测,评估其作为皮内免疫佐剂的潜力。结果显示,两种佐剂均无红肿破损等皮肤副反应,可引起皮肤短暂性的炎性因子上调和炎性细胞聚集,Gel02相较ISA15皮肤吸收性更快。两种佐剂均能显著提升血清中血凝抑制和IgG及其亚类抗体效价。Gel02倾向诱导IgG2a和IgG2c反应,ISA15则诱导IgG1反应。Gel02能提升肺泡灌洗液中IgG表达,不能提升IgA表达,ISA15均不能提升。因此,Gel02皮内免疫安全性、吸收性和效力均良好,更适用于猪流感皮内免疫,为猪流感灭活苗皮内免疫新技术研究奠定了基础。Intradermal immunity is an efficient immune route for vaccine administration.There is an urgent need of safe and efficient adjuvants for intradermal immunity.In this study,commercial adjuvants Gel02 and ISA15 were used to develop swine influenza virus(SIV)inactivated vaccines,and the local safety of skin and immune efficacy were evaluated in Balb/c mice.The local reactions of skin were examined from 0-8 d,then the antibodies in serum and bronchoalveolar lavage fluid(BALF)were detected on 28d.The results indicated that neither of the adjuvants caused significant local skin reactions,such as redness,swelling and breakage.Meanwhile,Gel02 and ISA15 both induced temporary up-regulation of inflammatory cytokines and aggregation of inflammatory cells in the skin.In addition,Gel02 was completely absorbed as early as 24h,which was faster than ISA15.Hemagglutination inhibition(HAI)and enzyme linked immunosorbent assay(ELISA)results indicated that ISA15 and Gel02 adjuvanted influenza vaccines induced higher level of antibodies compared to H3N2 alone.Gel02 tended to elicit IgG2c and IgG2a antibodies.However,ISA15 tended to elicit IgG1 antibodies.In BALF,Gel02 adjuvanted vaccine induced higher level of IgG antibodies,but not IgA antibodies.However,ISA15 could not induce the expression of IgG and IgA antibodies in BALF.In summary,the intradermal immune efficacy and safety of Gel02 and ISA15 were first confirmed in this study,which indicated that Gel02 was more suitable for intradermal immunity of SIV inactivated vaccines.This study established an important foundation for the development of swine influenza intradermal vaccines in the future.

关 键 词：皮内免疫 佐剂 猪流感 安全性 效力 

分 类 号：S852.659.5[农业科学—基础兽医学] S858.28[农业科学—兽医学]