养基鉴定津巴布韦烟草叶面优势微生物群落  

Identification of Dominant Microbial Communities on the Foliage of Zimbabwean Tobacco Using a Novel Tobacco-containing Medium

作　　者：邱燕玲 周瑢[2] 朱鑫权 徐艺雯 陶红[2] 潘晓薇[2] 欧阳璐斯[2] QIU Yan-ling;ZHOU Rong;ZHU Xin-quan(School of Life Sciences,Sun Yat-sen University,Guangzhou,Guangdong 510275;Technology Center,China Tobacco Guangdong Industrial Co.,Ltd.,Guangzhou,Guangdong 510385)

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]广东中烟工业有限责任公司技术中心,广东广州510385

出　　处：《安徽农业科学》2025年第8期1-6,共6页Journal of Anhui Agricultural Sciences

基　　金：广东中烟工业有限责任公司和中山大学横向科研项目(粤烟工2022科字第29号)。

摘　　要：为更好地鉴定津巴布韦烟草叶面的优势微生物,以LB固体培养基为基础,通过分别添加浓度(体积分数)为0%、2.5%、5.0%、10.0%的烟草成分以模拟烟叶表面微生物生长环境,筛选适合微生物生长的烟叶浓度,摸索合适的烟草培养基制备条件,分析不同浓度烟叶的微生物群落组成差异,分离鉴定特有的微生物。结果表明,浓度为2.5%和5.0%的烟叶培养基在灭菌后调节pH至7.0时,可分离出更多的特有微生物群落。通过宏基因组测序技术获得津巴布韦烟叶表面微生物的群落分布情况,发现芽孢杆菌属(Bacillus)在浓度为0%、2.5%、5.0%的培养基中有很高的相对丰度,而添加烟草后培养基中土地芽孢杆菌属(Terribacillus)成为优势种。在2.5%和5.0%烟草培养基上相对丰度较高的属有鱼芽孢杆菌属(Piscibacillus)、双芽孢杆菌属(Amphibacillus)、Margalitia等。通过分离测序,鉴定出15种烟叶表面优势微生物,如芽孢杆菌属的芽孢杆菌、贝莱斯芽孢杆菌、枯草芽孢杆菌等。In order to better identify the dominant microorganisms on the leaves of Zimbabwean tobacco,tobacco components at concentrations(volume fractions)of 0%,2.5%,5.0%and 10.0%were added to simulate the microbial growth environment on the surface of tobacco leaves,to screen the concentration of tobacco leaves suitable for microbial growth,to explore suitable tobacco culture medium preparation conditions,to analyze the differences in the composition of the microbial communities of tobacco leaves at different concentrations,to isolate and identify specific microorganisms.The results showed that more specific microbial communities could be isolated when the tobacco leaf culture medium was adjusted to pH 7.0 at concentrations of 2.5%and 5.0%after sterilization.Metagenomic sequencing technology was used to determine the community distribution of microorganisms on the surface of tobacco leaves in Zimbabwe.It was found that Bacillus had a high relative abundance in the culture medium at concentrations of 0%,2.5%and 5.0%,and after the addition of tobacco to the culture medium,Terribacillus became the dominant species.The genera with higher relative abundance on tobacco culture medium at concentrations of 2.5%and 5.0%included Piscibacillus,Amphibacillus,Margalitia,etc.Through isolation and sequencing,15 kinds of dominant microorganisms on the surface of tobacco leaves were identified,such as Bacillus species of the genus Bacillus,Bacillus veleis and Bacillus subtilis.

关 键 词：烟草培养基 津巴布韦烟叶 宏基因组 优势微生物群落 

分 类 号：TS414[农业科学—烟草工业]