基于HIF-1α通路探讨中药肾康灵对CKD大鼠肾脏氧化应激和纤维化程度的影响  

Exploring the Effects of Traditional Chinese Medicine Shenkangling on the Degree of Renal Oxidative Stress and Fibrosis in Rats with Chronic Kidney Disease Based on the HIF-1αPathway

作　　者：黄润宇 黎婷 范雪琪 庄翔莉 郑建 HUANG Runyu;LI Ting;FAN Xueqi;ZHUANG Xiangli;ZHENG Jian(Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350004,China;Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学附属人民医院,福建福州350004 [2]福建中医药大学,福建福州350122

出　　处：《中国民族民间医药》2025年第8期24-28,39,共6页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：福建省自然科学基金面上项目(No.2021J01900);福建省卫生健康青年科研课题(No.2023QNA077);国家中医药管理局高水平中医药重点学科建设项目-中西结合临床(No.zyyzdxk-2023104)。

摘　　要：目的:观察中药肾康灵对慢性肾脏病大鼠模型中肾脏氧化应激状态及肾脏纤维化进程的影响,并据此阐述其对慢性肾脏病所致肾损伤潜在的保护机制。方法:30只疾病稳定SD大鼠随机分为正常组、模型组和治疗组,每组10只。通过腺嘌呤诱导法成功构建慢性肾脏病大鼠模型后,对治疗组大鼠进行肾康灵药液(剂量为1.5 g/kg)灌胃治疗,持续干预28 d。利用生化分析仪测定各组大鼠血清中的尿素氮和血肌酐含量;采用HE染色观察小鼠肾组织病理形态;借助生化试剂盒检测肾脏组织中MDA、SOD及GSH-Px的表达水平;运用Western blot技术评估HIF-1α作为缺氧标志物的表达,以及纤维化相关蛋白α-SMA和TGF-β1的蛋白表达量。结果:与正常组比较,模型组大鼠肾脏血尿素氮和血肌酐含量显著增加(P<0.05),MDA、HIF-1α、TGF-β1表达显著升高(P<0.05),SOD、GSH-Px表达显著降低(P<0.05);与模型组比较,治疗组大鼠血尿素氮和血肌酐含量明显减少(P<0.05),MDA、HIF-1α、TGF-β1明显降低(P<0.05),SOD、GSH-Px表达显著增加(P<0.05)。结论:中药肾康灵可能通过调控HIF-1α信号通路,有效减轻慢性肾脏病大鼠的氧化应激状态,进而缓解肾脏纤维化过程,为延缓CKD疾病进展提供了一种潜在的治疗策略。Objective To investigate the effects of Shenkangling,a traditional Chinese medicine,on renal oxidative stress status and renal fibrosis progression in a rat model of chronic kidney disease(CKD),and to elucidate its potential protective mechanism against CKD-induced renal injury.Methods A total of thirty Sprague-Dawley(SD)rats were randomly assigned to three distinct experimental groups in an equal distribution of ten rats per group:a normal control group,a chronic kidney disease(CKD)model group,and a treatment group receiving Shenkangling intervention.After successfully establishing the CKD rat model through adenine induction,rats in the treatment group were administered with Shenkangling liquid(1.5 g/kg)via gavage for 28 consecutive days.Biochemical analyzer was used to determine the serum levels of blood urea nitrogen and serum creatinine in rats from each group.HE staining was used to observe the pathological morphology of mouse kidney tissue To quantify the expression levels of oxidative stress biomarkers in renal tissues,specialized biochemical kits were utilized,focusing on the measurement of malondialdehyde(MDA)as an indicator of lipid peroxidation,superoxide dismutase(SOD)as a primary antioxidant enzyme,and glutathione peroxidase(GSH-Px)as a key component of the glutathione-based antioxidant system.Furthermore,Western blot analysis was conducted to evaluate the protein expression of hypoxia-inducible factor-1α(HIF-1α),serving as a pivotal marker of hypoxia within the renal microenvironment.Additionally,the analysis encompassed the assessment of fibrosis-related proteins,specificallyα-smooth muscle actin(α-SMA)and transforming growth factor-β1(TGF-β1).Results Compared with the normal group,rats in the model group exhibited significantly increased levels of blood urea nitrogen and serum creatinine in the kidney(P<0.05),along with elevated expressions of MDA,HIF-1α,and TGF-β1(P<0.05),while the expressions of SOD and GSH-Px were significantly decreased(P<0.05).In contrast,rats in the treatment group s

关 键 词：慢性肾脏病 HIF-1Α 氧化应激 

分 类 号：R285.5[医药卫生—中药学]