维药茴芹果药材中异荭草苷的含量测定  

Determination of the Content of Isoanthocyanin in the Uyghur Medicine Pimpinellae Anisi Fructus

作　　者：王贺伟[1] 尹强 尹海龙 穆丹丹 田芳 李超杰 WANG Hewei;YIN Qiang;YIN Hailong;MU Dandan;TIAN Fang;LI Chaojie(Shihezi University,Shihezi 832003,China;Xinjiang Uighur Pharmaceutical Co.,Ltd.,Urumqi 830026,China)

机构地区：[1]石河子大学,新疆石河子832003 [2]新疆维吾尔药业有限责任公司,新疆乌鲁木齐830026

出　　处：《中国民族民间医药》2025年第8期56-58,106,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：新疆地产药材配方颗粒研制及中试技术平台搭建(编号:2022LQ03020);新疆维吾尔自治区“天山英才”-优秀工程师培养计划项目。

摘　　要：目的:建立茴芹果中异荭草苷的含量测定方法。方法:利用HPLC法,以Aglient-ZORBAX-Eclipse-Plus C_(18)柱为色谱柱,使用甲醇-0.1%磷酸为流动相(梯度洗脱),检测波长为347 nm,测定茴芹果药材中异荭草苷的含量。结果:异荭草苷浓度在3.625~64.108μg/mL范围时,峰面积与浓度呈良好的线性关系(R^(2)=0.9994),平均加样回收率101.8408%(RSD=3.5%,n=9)。异荭草苷含量为292.1803~491.4936μg/g。结论:建立的含量测定方法,容易操作,可控性良好,适用于茴芹果的质量控制。Objective Establish a method for determining the content ofisoorientin in Pimpinellae anisi fructus.Method High-performance liquid chromatography(HPLC)was employed using an Agilent ZORBAX Eclipse Plus C_(18)column,with a mobile phase of methanol-0.1%phosphoric acid(gradient elution).Detection was conducted at a wavelength of 347nm to determine the content of isoorientin in Pimpinellae anisi fructus.Results A good linear relationship was observed between the peak area and concentration of isoorientin in the range of 3.625 to 64.108μg/g(R^(2)=0.9994).The average sample recovery rate was 101.8408%(RSD=3.5%,n=9).The content of isoorientin ranged from 292.1803 to 491.4936μg/g.Conclusion The established determination method is easy to operate with good controllability,suitable for quality control of Pimpinellae anisi fructus.

关 键 词：茴芹果 异荭草苷 高效液相色谱 

分 类 号：R281.1[医药卫生—中药学]