指纹图谱结合含量测定分析黄芪闷润和蒸润切制的量值传递规律  

Analysis of the quantity transfer rules in processing of Astragalus membranaceus by moistening-soaking and steaming-soaking followed by cutting based on fingerprint combined with content determination

作　　者：赵晨光 汤书婉 刘顺 吴旭彤 彭国平[2,3] 李存玉 ZHAO Chenguang;TANG Shuwan;LIU Shun;WU Xutong;PENG Guoping;LI Cunyu(Dept.of Preparation,the Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine,Nanjing 210029,China;College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing 210023,China)

机构地区：[1]南京中医药大学附属医院/江苏省中医院制剂部,南京210029 [2]南京中医药大学药学院,南京210023 [3]江苏省中药资源产业化过程协同创新中心,南京210023

出　　处：《中国药房》2025年第9期1065-1070,共6页China Pharmacy

基　　金：江苏省自然科学基金面上项目(No.BK20211395);江苏省中医药科技发展计划面上项目(No.MS2022019);南京市医疗机构中药传统制剂立项项目(No.NJCC-ZJ-202343);江苏省中医疫病研究中心开放课题(No.JSYB2024KF11);江苏省中医院院内课题(No.Y24061)。

摘　　要：目的分析黄芪闷润、蒸润切制前后的量值传递规律。方法制备3批黄芪闷润、蒸润切制饮片。通过《中药色谱指纹图谱相似度评价系统(2012版)》建立黄芪药材及其闷润、蒸润切制饮片的高效液相色谱叠加指纹图谱,结合前期定性分析结果指认共有峰,分析共有峰峰面积变化,并进行主成分分析。采用高效液相色谱法测定黄芪药材及其闷润、蒸润切制饮片中毛蕊异黄酮苷、黄芪皂苷Ⅰ、黄芪甲苷的含量,并比较不同样品中各成分的含量差异。结果指纹图谱分析结果显示,共指认了17个共有峰;黄芪闷润、蒸润后,饮片中各共有峰峰面积占比相比药材均发生了变化(如黄芪蒸润切制饮片中丙二酰基毛蕊异黄酮苷和丙二酰基黄芪皂苷Ⅰ峰面积占比降低,毛蕊异黄酮苷峰面积占比升高)。主成分分析结果显示,黄芪药材、黄芪闷润切制饮片、黄芪蒸润切制饮片各自聚为一类。含量测定结果显示,与黄芪药材相比,黄芪闷润切制饮片中毛蕊异黄酮苷的平均含量显著降低(P<0.05);黄芪蒸润切制饮片中毛蕊异黄酮苷、黄芪甲苷的平均含量均显著升高(P<0.05);黄芪闷润切制饮片中黄芪甲苷以及2种饮片中黄芪皂苷Ⅰ的平均含量差异均无统计学意义(P>0.05)。结论黄芪药材闷润、蒸润切制前后量值传递规律存在差异,其中蒸润切制可能使不稳定成分(如丙二酰基毛蕊异黄酮苷、丙二酰基黄芪皂苷Ⅰ)转化更完全。OBJECTIVE To analyze the quantity transfer rule in the processing of Astragalus membranaceus before and after moistening-soaking and steaming-soaking followed by cutting.METHODS Three batches of A.membranaceus decoction pieces processed through moistening-soaking and steaming-soaking followed by cutting were prepared.The HPLC overlapping fingerprints of A.membranaceus and its decoction pieces were established through the Similarity Evaluation System of Chromatographic Fingerprints of TCM(2012 edition).Combined with the previous qualitative analysis results,the common peaks were identified,the changes of common peak area were analyzed,and the principal component analysis was carried out.The contents of calycosin-7-glucoside,astragalosideⅠand astragalosideⅣin A.membranaceus and its decoction pieces were determined by HPLC,and the content differences of each component in different samples were compared.RESULTS The results of fingerprint analysis showed that 17 common peaks were identified.After steaming-soaking and moistening-soaking of A.membranaceus,the proportion of common peak area in the decoction pieces changed compared with the original medicine(for example,in A.membranaceus steaming-soaking decoction pieces,the proportion of peak area of malonyl calycosin-7-glucoside and malonyl astragalosideⅠdecreased,while the proportion of peak area of calycosin-7-glucoside increased).The results of principal component analysis showed that A.membranaceus,and its decoction pieces after moistening-soaking and steaming-soaking followed by cutting were all clustered into one category respectively.The results of content determination showed that,compared with A.membranaceus,the average content of calycosin-7-glucoside in A.membranaceus moistening-soaking decoction pieces was significantly reduced(P<0.05);the average contents of calycosin-7-glucoside and astragalosideⅣin A.membranaceus steamingsoaking decoction pieces were significantly increased(P<0.05);there was no significant difference in the average content of astr

关 键 词：黄芪 闷润切制 蒸润切制 指纹图谱 含量测定 量值传递规律 毛蕊异黄酮苷 黄芪皂苷Ⅰ 黄芪甲苷 

分 类 号：R917[医药卫生—药物分析学]