不同基原枳实的多组分含量测定及差异性成分分析  

Determination of multi-component content and analysis of differential components in Aurantii Fructus Immaturus from different origins

作　　者：吴倩颖 刘德鸿 陈伟康 陈希 朱艳艳 王杰 WU Qianying;LIU Dehong;CHEN Weikang;CHEN Xi;ZHU Yanyan;WANG Jie(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Jiangxi Institute for Drug Control/NMPA Key Laboratory of Quality Evaluation of Chinese Patent Medicine/Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Nanchang 330029,China)

机构地区：[1]江西中医药大学药学院,南昌330004 [2]江西省药品检验检测研究院/国家药品监督管理局中成药质量评价重点实验室/江西省药品与医疗器械质量工程技术研究中心,南昌330029

出　　处：《中国药房》2025年第9期1071-1075,共5页China Pharmacy

基　　金：江西省重点研发计划项目(No.20212BBG73025);江西省药品监督管理局科研项目(No.2021KY44)。

摘　　要：目的测定不同基原(酸橙、香橙、臭橙、脐橙、甜橙)枳实中15种成分的含量,筛选差异性成分,为枳实的质量评价提供参考。方法采用高效液相色谱法对46批不同基原枳实中辛弗林、N-甲基酪胺、5,7-二羟基色酮-7-新橙皮糖苷、新北美圣草苷、柚皮芸香苷、柚皮苷、橙皮苷、新橙皮苷、柚皮素、橙皮素、甜橙黄酮、川陈皮素、橘皮素、5-去甲川陈皮素、橙皮油素的含量进行测定。色谱条件采用Waters Symmetry C_(18)色谱柱,以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱;流速为1.0 mL/min;柱温为40℃;检测波长为284 nm;进样量为5μL。采用聚类分析、主成分分析(PCA)、正交偏最小二乘-判别分析(OPLS-DA)法分析不同基原枳实样品间的差异,并筛选差异性成分。结果上述15种成分在其检测质量浓度范围内线性关系均良好(r均大于0.9990),精密度、稳定性(24 h)及重复性试验的RSD均小于2.00%,平均加样回收率为91.1%~103.9%(RSD均小于3.10%)。聚类分析、PCA、OPLS-DA结果显示,甜橙、脐橙基原枳实聚为一类,酸橙、香橙、臭橙基原枳实聚为另一类;新橙皮苷、橙皮油素、柚皮苷、新北美圣草苷、橘皮素、橙皮苷、甜橙黄酮、5,7-二羟基色酮-7-新橙皮糖苷的变量重要性投影值大于1。结论本研究测定了不同基原枳实中15种成分的含量,并筛选出了包括新橙皮苷、橙皮油素、柚皮苷、新北美圣草苷等在内的8种差异性成分。OBJECTIVE To determine the contents of 15 components in Aurantii Fructus Immaturus from different origins(Citrus aurantium,C.junos,C.aurantium Linn.,C.sinensis Osb.,C.sinensis),screen differential components,and provide references for the quality evaluation of Aurantii Fructus Immaturus.METHODS HPLC method was adopted to determine the contents of synephrine,N-methyltyramine,5,7-dihydroxychromone-7-neohesperidoside,neoponcirin,narirutin,naringin,hesperidin,neohesperidin,naringenin,hesperetin,sinensetin,nobiletin,tangeretin,5-demethylnobiletin,and auraptene in 46 batches of Aurantii Fructus Immaturus from different origins.The determination was performed on Waters Symmetry C_(18)column with mobile phase consisted of acetonitrile-0.1%formic acid(gradient elution)at the flow rate of 1.0 mL/min;column temperature was set at 40℃,detection wavelength was 284 nm,and sample injection volume was 5μL.The differences between different origins of Aurantii Fructus Immaturus were analyzed by cluster analysis,principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA),and differential components were screened.RESULTS The linear relationships of the aforementioned 15 components were all good within the tested mass concentration ranges(all r>0.9990).The RSDs for precision,stability(24 h),and repeatability tests were all less than 2.00%.The average recovery rate ranged from 91.1%to 103.9%(all RSDs<3.10%).Cluster analysis,PCA,and OPLS-DA revealed that C.sinensis Osb.and C.sinensis were clustered into one category,while C.aurantium,C.junos and C.aurantium Linn.were clustered into another category.The variable importance projection values for neohesperidin,auraptene,naringin,neoponcirin,tangeretin,hesperidin,sinensetin,and 5,7-dihydroxychromone-7-neohesperidoside were all greater than 1.CONCLUSIONS In this study,the contents of 15 components in Aurantii Fructus Immaturus from different origins are determined,and 8 differential components,including neohesperidin,auraptene,naringin,and neoponc

关 键 词：枳实 基原 多元统计分析 含量测定 差异性成分 

分 类 号：R917[医药卫生—药物分析学]