基于血小板代谢组学探究4℃冷藏对单采血小板保存的影响  

Research the effect of 4℃refrigerated stored apheresis platelets based on platelet metabolomics

作　　者：夏小叶 李雪晶 苏爱华 郝萧 叶红艳 XIA Xiaoye;LI Xuejing;SU Aihua;HAO Xiao;YE Hongyan(Jinan Blood Center,Jinan 250000,China;Jinan Stomatological Hospital,Jinan 250000,China)

机构地区：[1]济南市血液供保中心,山东济南250001 [2]济南市口腔医院,山东济南250001

出　　处：《中国输血杂志》2025年第4期514-521,共8页Chinese Journal of Blood Transfusion

基　　金：济南市卫生健康委员会科技计划项目(2023-1-64)。

摘　　要：目的探究单采血小板于4℃冷藏保存与22℃振荡保存在代谢组学上的差异,为单采血小板的冷藏保存提供理论依据。方法对4℃冷藏保存血小板(实验组)d1、d5、d10、d154个时间点和22℃振荡保存血小板(对照组)d1、d52个时间点采集标本,用液质联用(LC-MS/MS)技术检测不同保存条件下的血小板代谢组水平的变化。采用血栓弹力图检测TEG-MA值与流式细胞仪检测血小板表面的CD62P活化率。结果糖代谢通路的多种代谢物、三羧酸循环中的关键代谢物(柠檬酸、α-酮戊二酸)、嘌呤代谢途径中的代谢物(腺嘌呤、腺苷次黄嘌呤核苷、鸟嘌呤等)与氨基酸代谢产物在对照组保存d5时含量显著降低,而在实验组中相对稳定。脂肪酸代谢产物如前列腺素G2、13(S)-HOTrE、亚油酸等含量在对照组显著升高。保存d1与d5时MA值对照组与实验组比较有差异(P<0.05),实验组随保存时间延长,MA值d10与d15时实验组与对照组d5比较无差异(P>0.05)。2组间CD62P活化率比较有差异(P<0.05),CD62P活化率对照组从d1起快速升高,而实验组升高缓慢。结论4℃冷藏保存的血小板代谢活动更为稳定和缓慢,有利于延长血小板的保存期。Objective To investigate the differences in metabolomics between apheresis platelets stored at 4℃and at 22℃with agitation,aiming to provide a theoretical basis for the cold storage of apheresis platelets.Methods Samples were collected at four time points(d1,d5,d10,d15)for platelets stored at 4℃(experimental group)and two time points(d1,d5)for platelets stored at 22℃with agitation(control group).Liquid chromatography-tandem mass spectrometry(LC-MS/MS)technology was used to detect changes in platelet metabolome levels under different storage conditions.Platelet functional activity was assessed by thromboelastography(TEG)for maximum amplitude(MA)values and flow cytometry for CD62P activation rates.Results Metabolites in the glycolytic pathway,key metabolites in the tricarboxylic acid cycle(citrate,α-ketoglutarate),metabolites in the purine metabolism pathway(adenine,inosine monophosphate,guanine,etc.)and amino acid metabolites significantly decreased by d5 in the control group,whereas they remained stable in the experimental group.The content of fatty acid metabolites,such as prostaglandin G2,13(S)-HOTrE,and linoleic acid,significantly increased in the control group.Statistically significant differences in MA values were observed between the two groups at d1 and d5(P<0.05).However,in the experimental group,as the storage time extended,the MA values at d10 and d15 showed no significant difference compared to the control group at d5(P>0.05).The CD62P activation rate between the two groups was statistically significant(P<0.05).Additionally,the CD62P activation rate of platelets in the 22℃group increased rapidly from d1,while it rose gradually in the 4℃group.Conclusion Platelets stored at 4℃exhibit more stable metabolic activity and slower functional deterioration,which is beneficial for extending the effective storage period of platelets.

关 键 词：冷藏保存 单采血小板 代谢组学 

分 类 号：R457.1[医药卫生—治疗学] R331.143[医药卫生—临床医学]