妊娠糖尿病对子代大鼠空腹血糖的影响及2'-岩藻糖基乳糖的干预效果研究  

Effects of gestational diabetes mellitus on fasting plasma glucose in offspring rats and the intervention effect of 2'-fucosyllactose

作　　者：叶湾韵 史涵旭 周雅琳 文彰 彭译乐 许雅君[1] Ye Wanyun;Shi Hanxu;Zhou Yalin;Wen Zhang;PengYile;Xu Yajun(Department of Nutrition and Food Hygiene,School of Public Health,Peking University.Beijing Key Laboratory for Research and Evaluation of Food Safety Toxicology,Peking University)

机构地区：[1]北京大学公共卫生学院营养与食品卫生学学系,食品安全毒理学研究与评价北京市重点实验室,100191

出　　处：《中国生育健康杂志》2025年第3期201-207,共7页Chinese Journal of Reproductive Health

基　　金：国家自然科学基金(82173500)。

摘　　要：目的研究妊娠糖尿病对大鼠子代空腹血糖的影响,探索2'-岩藻糖基乳糖(2'-FL)改善妊娠糖尿病子代空腹血糖受损的能力及适宜剂量。方法将健康SD大鼠随机分为空白对照组及妊娠糖尿病(GDM)造模组。以高脂饮食配合小剂量链脲佐菌素腹腔注射建立GDM模型,GDM造模组产后随机分为对照组(GDM组)、GDM+2'-FL低剂量干预组(GLF组)、GDM+2'-FL中剂量干预组(GMF组)、GDM+2'-FL高剂量干预组(GHF组),每组8只孕鼠。于产后第3~21天对仔鼠进行灌胃,空白对照组与GDM组灌胃蒸馏水,GLF组、GMF组和GHF组分别给予0.2 g/kg、0.6 g/kg和1.8 g/kg的2'-FL灌胃。观察记录母鼠孕期、哺乳期空腹血糖和体重,无菌采集产后第3、11、21天乳汁分析2'-FL含量。记录仔鼠哺乳期体重,于产后第21天检测空腹血糖,产后第7、21天每窝处死雌雄各一只仔鼠,称取主要脏器重量并计算脏器系数。结果GDM组母鼠产后第3、21天乳汁中2'-FL含量显著低于空白对照组(P<0.05),两组乳汁中2'-FL含量在哺乳期均随时间推移降低。GDM组雌、雄仔鼠产后第21天空腹血糖均较空白对照组显著升高(P<0.05),GLF组的雌、雄仔鼠空腹血糖较GDM组显著降低(P<0.05),GMF组和GHF组与GDM组比较,仅雄性仔鼠空腹血糖显著降低(P<0.05)。产后第7天雌、雄仔鼠肝脏系数较空白对照组显著降低(P<0.05),GHF组雌鼠肝脏系数较GDM组显著升高(P<0.05)。产后第21天GDM组雄性仔鼠肝脏系数和雌性仔鼠肾脏系数较空白对照组显著升高(P<0.05),GHF组雌性仔鼠肾脏系数较GDM组显著降低(P<0.05)。结论受妊娠糖尿病影响的仔鼠空腹血糖受损,2'-FL干预可在一定程度上改善仔鼠空腹血糖受损。Objective To study the effect of gestational diabetes mellitus(GDM)on fasting blood glucose in offspring,and to explore the ability of 2'-fucosyllactose(2'-FL)to improve impaired fasting blood glucose in offspring with GDM and the appropriate dose.Methods Healthy SD rats were randomly divided into control group and GDM model group.The GDM model was established by high-fat diet combined with low-dose streptozotocin intraperitoneal injection.The GDM model group was randomly divided into GDM model group(GDM group),GDM+2'-FL low-dose intervention group(GLF group),GDM+2'-FL medium-dose intervention group(GMF group),GDM+2'-FL high-dose intervention group(GHF group).There were 8 pregnant mice in each group.The offspring rats in the control group and GDM group were given distilled water by gavage,and the rats in the 2'-FL intervention groups were given 2'-FL at 0.2 g/kg,0.6 g/kg and 1.8 g/kg,respectively.Fasting blood glucose(FPG)and body weight of maternal rats during pregnancy and lactation were recorded.Breast milk samples were collected on postnatal days 3,11 and 21 for analysis of 2'-FL content.The body weight of offspring rats during lactation was recorded,and FPG was measured on the 21st day after delivery.On the 7th and 21st day after delivery,one male and one female offspring of each litter were sacrificed to weigh the main organs and calculate the organ coefficient.Results The content of 2'-FL in breast milk of the GDM model group was significantly lower than that of the control group on postnatal days 3 and 21(P<0.05),and the content of 2'-FL in breast milk of the two groups decreased over time during lactation.The FPG of female and male offspring in the GDM group was significantly higher than that in the control group on postnatal day 21(P<0.05),and the FPG of female and male offspring in the low-dose 2'-FL intervention group was significantly lower than that in the GDM group(P<0.05).The FPG of male offspring in the medium-and high-dose 2'-FL intervention groups was significantly lower than that in the contro

关 键 词：妊娠糖尿病 2′-岩藻糖基乳糖 干预作用 空腹血糖受损 

分 类 号：R17[医药卫生—妇幼卫生保健] R71[医药卫生—公共卫生与预防医学]