SSBP1对卵巢癌细胞自噬和凋亡的影响及作用机制分析  

Effect and mechanism analysis of SSBP1 on autophagy and apoptosis of ovarian cancer cells

作　　者：苗丽 王栋[1] 王颖[2] 马莉[1] 康安静[1] MIAO Li;WANG Dong;WANG Ying;MA Li;KANG Anjing(Department of Pathology,the Second Affiliated Hospital of Xi′an Jiaotong University;Department of Obstetrics and Gynecology)

机构地区：[1]西安交通大学第二附属医院病理科,710004 [2]西安交通大学第二附属医院妇产科,710004

出　　处：《中国生育健康杂志》2025年第3期220-225,共6页Chinese Journal of Reproductive Health

基　　金：陕西省科学技术研究发展计划项目(编号:2012SF2-03-04)。

摘　　要：目的探究SSBP1基因对卵巢癌细胞自噬和凋亡的影响和作用机制。方法研究对象选取卵巢癌耐药细胞系SKOV3细胞例,培养后分为39个样本,分为常规培养组、SSBP1抑制剂组、SSBP1模拟物组,每组13例,观察各组细胞活力、细胞增殖率、细胞凋亡率、细胞自噬相关蛋白、细胞凋亡相关蛋白及CHOP、DCR、Caspase8蛋白相对表达。结果SSBP1抑制剂组细胞活力、细胞增殖率、LC3I、Bcl-2蛋白相对表达低于常规培养组,细胞凋亡率、p62、LC3II、Bax、CHOP、DCR、Caspase8、active-Caspase8、cleaved-Caspase8蛋白及SSBP1基因相对表达高于常规培养组,SSBP1模拟物组细胞活力、细胞增殖率、LC3I、Bcl-2蛋白相对表达显高于常规培养组,细胞凋亡率、p62、LC3II、Bax、CHOP、DCR、Caspase8、active-Caspase8、cleaved-Caspase8蛋白及SSBP1基因相对表达低于常规培养组(P<0.05)。结论SSBP1基因能调节卵巢癌细胞凋亡相关蛋白表达,其低表达促进卵巢癌细胞凋亡,抑制卵巢癌细胞自噬、细胞活力,分析其作用机制可能与CHOP/DCR/Caspase8信号通路有关,其有望成为卵巢癌治疗新的药物靶点。Objective To explore the effect and mechanism of SSBP1 gene on autophagy and apoptosis of ovarian cancer cells.Methods The subjects of the study selected drug-resistant ovarian cancer cell line SKOV3 cell line and were divided into 39 samples after culture,13 of which were used as the control group for routine culture,and the other 26 were used as the study group,which were respectively given SSBP1 inhibitor(13 strains)and SSBP1 active(13 strains)intervention.Cell viability,cell proliferation rate,cell apoptosis rate,autophagy related proteins,apoptosis related proteins,CHOP,DCR,Caspase8 proteins were observed in each group.Results Cell viability,cell proliferation rate,LC3I and Bcl-2 protein relative expression in SSBP1 inhibitor 1 were lower than those in control group.Apoptosis rate,p62,LC3II,Bax,CHOP,DCR,Caspase8,active-Caspase8,cleaved-Caspase8 protein and relative expression of SSBP1 gene were higher than those in the control group.Cell viability,cell proliferation rate,LC3I and Bcl-2 protein relative expression in SSBP1 inhibitor 2 were significantly higher than those in control group.Apoptosis rate,p62,LC3II,Bax,CHOP,DCR,Caspase8,active-Caspase8,cleaved-Caspase8 protein and relative expression of SSBP1 gene were lower than those in control group(P<0.05).Conclusion SSBP1 gene can regulate the expression of apoptosis-related proteins in ovarian cancer cells,and its low expression can promote the apoptosis of ovarian cancer cells,inhibit the autophagy and cell viability of ovarian cancer cells.The mechanism of SSBP1 gene may be related to the CHOP/DCR/Caspase8 signaling pathway,and it is expected to become a new drug target for ovarian cancer treatment.

关 键 词：SSBP1 卵巢癌 CHOP DCR CASPASE8 自噬 凋亡 

分 类 号：R71[医药卫生—妇产科学]