复方芪芎颗粒对类风湿性关节炎模型大鼠滑膜趋化因子的调控作用研究  

Study on the regulatory effect of Fu-fang-qi-qiong granule on synovial chemokines in rheumatoid arthritis model rats

作　　者：陈利锋 张钰景 卢绮萍 陈芳 谭张奎 CHEN Lifeng;ZHANG Yujing;LU Jiping(Department of Rheumatology and Immunology,Central Theater General Hospital of Chinese People's Liberation Army,Wuhan 430070,China;General surgery department,Central Theater General Hospital of Chinese People's Liberation Army,Wuhan 430070,China)

机构地区：[1]中国人民解放军中部战区总医院风湿免疫科,湖北武汉430070 [2]中国人民解放军中部战区总医院普外科,湖北武汉430070 [3]中国人民解放军中部战区总医院医疗保障中心实验动物室,湖北武汉430070 [4]湖北省中医院风湿病科,湖北武汉430030

出　　处：《四川中医》2025年第3期121-125,共5页Journal of Sichuan of Traditional Chinese Medicine

基　　金：湖北省医学青年拔尖人才(鄂卫通[2019] 48号);武汉市科技局知识创新专项项目(编号:2022020801010519);中部战区总医院博士后科研启动基金(编号:20211015KY19)。

摘　　要：目的:探讨复方芪芎颗粒调控MCP1,CCR2蛋白调控炎症因子抗类风湿性关节炎(RA)的机制。方法:将32只SD大鼠随机分为空白组、模型组、中药组、甲氨蝶呤组4组。通过CIA法制备RA模型,造模14天后每3天评价关节炎指数并检测踝关节体积。6周后取材,通过HE染色检测各组大鼠踝关节病理改变,通过Elisa检测大鼠血清IL-6、TNF-α的含量,通过免疫荧光检测滑膜MCP-1、CCR2,通过WB检测滑膜组织IL-6、TNF-α蛋白的表达。结果:与正常组相比,模型组滑膜炎症反应明显,与模型组相比,中药组和甲氨蝶呤组炎症明显减轻;与正常组相比,模型组关节炎指数,关节肿胀度明显升高(P<0.05),血清IL-6、TNF-α及滑膜MCP1、CCR1、IL-6、TNF-α蛋白表达量显著升高(P<0.05);与模型组相比,中药组和甲氨蝶呤组均能降低大鼠关节炎指数和关节肿胀度(P<0.05),降低血清IL-6、TNF-α及滑膜MCP-1、CCR1、IL-6、TNF-α蛋白表达量(P<0.05)。结论:复方芪芎颗粒可以缓解RA模型大鼠踝关节炎症,这可能与调控趋化因子的表达,减少炎症因子浸润有关。Objective: To explore the mechanism of Fu-fang-qi-qiong granule regulating MCP1,CCR2protein regulating inflammatory factor anti-RA. Methods: 45SD rats were randomly divided into 4groups: blank group,model group,traditional Chinese medicine group and methotrexate group. The RA model was prepared by CIA method,and the arthritis index was evaluated and the ankle joint volume was detected every 3days after 14days of modeling. 6weeks later,the samples were taken and the pathological changes of the ankle joints of the rats in each group were detected by HE staining,and the levels of serum IL-6and TNF-α in rats were detected by Elisa,and the levels of synovial MCP-1and CCR2were detected by immunofluorescence,and the expressions of synovial tissues' IL-6and TNF-α protein expression in synovial tissue. Results: Compared with the normal group,the synovial inflammatory reaction was obvious in the model group,and the inflammation was significantly reduced in the traditional Chinese medicine group and methotrexate group compared with the model group;compared with the normal group,the arthritis index,joint swelling was significantly higher in the model group ( P <0.05),and the expression of serum IL-6,TNF-α,and synovial MCP1,CCR1,IL-6,and TNF-α proteins were significantly higher in the model group ( P <0.05). Compared with the model group,both the traditional Chinese medicine group and the methotrexate group reduced the arthritis index and joint swelling in rats ( P <0.05),and lowered the serum IL-6,TNF-α and synovial MCP-1,CCR1,IL-6,TNF-α protein expression ( P <0.05). Conclusion: Compound astragalus rhizome granules can alleviate ankle joint inflammation in RA model rats,which may be related to the modulation of chemokine expression to reduce the infiltration of inflammatory factors.

关 键 词：复方芪芎颗粒 MCP1 CCR2 炎症因子 

分 类 号：R285[医药卫生—中药学]