葛根芩连汤调节肠道菌群抑制大肠癌的作用机制研究  

Study on the mechanism of regulating intestinal flora of gegen qinlian decoction in inhibiting colorectal cancer

作　　者：谢青 林湘萍 伍鋆 骆小珊 谢甦 XIE Qing;LIN Xiangping;WU Yun(Affiliated Hospital of Guizhou Medical University,Guiyang 550001,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]贵州医科大学附属医院,贵州贵阳550001 [2]北京中医药大学东直门医院,北京100700 [3]贵州医科大学,贵州贵阳550001

出　　处：《四川中医》2025年第3期126-131,共6页Journal of Sichuan of Traditional Chinese Medicine

基　　金：贵州省中医药管理局中医药、民族医药科学技术课题(编号:QZYY-2023-003)。

摘　　要：目的:探究葛根芩连汤调节肠道微生态抑制大肠癌的作用机制。方法:将4周龄Wistar雄性大鼠随机分为三组:模型组、湿热型大肠癌组、中药(葛根芩连汤)+湿热型大肠癌组,构建湿热型大肠癌大鼠模型,给予葛根芩连汤干预28天,采用16S rDNA实时荧光定量PCR技术比较分析各大鼠大肠粪便中菌群的结构和多样性,运用免疫组织化学法分析各组肿瘤组织中Ki67、微血管密度(MVD),ELISA测定各组血清中血管内皮细胞生长因子(VEGF)、MMP2、MMP7、MMP9的表达。结果:与湿热型大肠癌组相比,葛根芩连汤干预后,大鼠肠道菌群结构和丰度均发生改变,并能降低MVD、Ki67的阳性率,下调VEGF、MMP2、MMP7、MMP9的表达(P<0.05)。结论:葛根芩连汤能够调节湿热型大肠癌大鼠肠道菌群的结构与丰度,对预防及治疗大肠癌复发转移有一定作用。Objective: To explore the mechanism of regulating intestinal microecology by Gegen Qinlian Decoction in inhibiting colorectal cancer. Methods: The 4-week-old Wistar male rats were randomly divided into three groups: model group,damp-heat type colorectal cancer group,traditional Chinese medicine (Gegen Qinlian Decoction) + damp-heat type colorectal cancer group,the damp-heat type colorectal cancer rat model was established,and Gegen Qinlian decoction was given for 28days. 16S rDNA real-time fluorescence quantitative PCR technique was used to compare and analyze the structure and diversity of bacteria in the feces of rats. Ki67and microvascular density (MVD) in tumor tissues of each group were analyzed by immunohistochemistry. The expressions of vascular endothelial growth factor (VEGF),MMP2,MMP7and MMP9in serum were determined by ELISA. Results: Compared with the damp-heat type colorectal cancer group,the intestinal flora structure and abundance of rats were changed after intervention with Gegenqinlian Decoction,and the positive rate of MVD and Ki67was decreased,and the expressions of VEGF,MMP2,MMP7and MMP9were down-regulated ( P <0.05). Conclusion: Gegenqinlian Decoction can regulate the structure and abundance of intestinal flora in damp-heat type rats with colorectal cancer,and play a certain role in preventing and treating recurrence and metastasis of colorectal cancer.

关 键 词：葛根芩连汤 肠道菌群 湿热证 大肠癌 

分 类 号：R285[医药卫生—中药学]