野苹果粉红聚端孢生物学特性及防控药剂筛选  

Study on the Biological Characteristics and Fungicide Screening of Trichothecium Roseum from Wild Apple Trees

作　　者：贾李智 陆永兴 王寒月 郭建伟 JIA Lizhi;LU Yongxing;WANG Hanyue;GUO Jianwei(Yunnan Provincial Key Laboratory of Konjac Biology(in planning)/Yunnan Provincial Engineering and Technology Research Center for Urban Characteristic Agriculture,Kunming University,Kunming Yunnan 650214,China;Xinjiang Institute of Ecology and Geography/State Key Laboratory of Desert and Oasis Ecology,Chinese Academy of Sciences,Urumqi Xinjiang 830011,China;School of Life Science,Shihezi University,Shihezi Xinjiang 832000,China)

机构地区：[1]昆明学院农学与生命科学学院/云南省魔芋生物学重点实验室(筹)/云南省都市特色农业工程技术研究中心,云南昆明650214 [2]中国科学院新疆生态与地理研究所/荒漠与绿洲生态国家重点实验室,新疆乌鲁木齐830011 [3]石河子大学生命科学学院,新疆石河子832000

出　　处：《保山学院学报》2025年第2期6-13,共8页JOURNAL OF BAOSHAN UNIVERSITY

基　　金：昆明学院引进人才项目“云南草果果腐病的病原多样性及致病性研究”(项目编号:XJ20230077);云南省教育厅科学研究基金研究生项目“缓解滇中烟草黑胫病AMF菌剂筛选及作用机制初探”(项目编号:2024Y731);云南省生物种业和农产品精深加工专项“怒江草果产业科技创新与应用研究”(项目编号:202202AE090035);国家重点研发计划项目“天山北坡退化野果林生态保育与健康调控技术”(项目编号:2016YFC0501500);新疆维吾尔自治区优秀博士后一般资助“新疆阿魏内生菌多样性及其季节动态”(项目编号:Y523131002);中国科学院特色研究所主要服务项目“天山重要生物资源就地和迁地保育原理与关键技术”(项目编号:TSS-2015-014-FW-4-2);新疆维吾尔自治区科技计划项目“天山野果林生态系统恢复与保育关键技术”(项目编号:2016B03040);新疆维吾尔自治区区域协同创新专项“中乌干旱荒漠区盐生植物促生细菌资源的挖掘及其应用潜力评估”(项目编号:2019E01016)。

摘　　要：粉红聚端孢(Trichothecium roseum)能引起栽培苹果霉心病、心腐病、黑点病及火棘枝枯病,探清其生物学特性有助于粉红聚端孢导致的病害的防控。从天山野果林的新疆野苹果枝枯病、心腐病样品分离到一株粉红聚端孢,采用菌丝生长速率法和血球计数板法,测试温度、pH值、光照等培养条件和碳源、氮源等营养条件对粉红聚端孢菌丝生长和产孢的影响,并筛选有效杀菌剂。探索天山野果林来源的粉红聚端孢生物学特性及有效杀菌剂。结果表明,粉红聚端孢XPK2-1菌丝生长的最适温度是25℃,最适pH值是7,光照促进菌丝生长效果不显著,最适碳氮源分别是淀粉、甘氨酸;产孢的最适温度是25℃,最适pH值是7,光照显著促进产孢,最适碳氮源分别是淀粉、硝酸钠。有效杀菌剂是氟硅唑,半致死浓度为7.791 mg/L。Trichothecium roseum can cause apple moldy core,core rot,black spot and pyracantha dieback,many strains of T.roseum were isolated from dieback and core rot on Malus sieversii(Ledeb.)M.Roem.from Tianshan wild apple forests.It would be benefit to prevent and control the diseases caused by T.roseum on M.sieversii that the biological characteristics were detected.The effect of cultural conditions such as temperature,light,pH value and nutrient such as carbon sources,nitrogen sources on mycelial growth and spore production of T.roseum XPK2-1,were tested by mycelial growth rate method and hemocytometer measurement,in order to detect the biological characteristics and fungicide of T.roseum from wild apple forests in Tianshan Mountain.The results show that the most suitable temperature for mycelial growth of T.roseum XPK2-1 was 25℃,the most suitable pH value was 7,light slightly promoted mycelial growth,and the most suitable carbon or nitrogen source was starch or glycine,respectively.The most suitable temperature for conidial production was 25℃,the most suitable pH value was 7,light significantly promoted conidial production,and the most suitable carbon or nitrogen source was starch or sodium nitrate,respectively.The most effective fungicide was flusilazole,the EC50 value was 7.791mg/L.

关 键 词：天山野果林 粉红聚端孢 生物学特性 防治药剂 半致死浓度 

分 类 号：Q1[生物学—普通生物学]