微全分析系统激光导向细胞分选方法  

Method of laser-guided cell sorting inμ-TAS

作　　者：周围[1] 郑泓飙 安广鑫 李志林 刘晓彤 王明慧 孟庆宜 ZHOU Wei;ZHENG Hongbiao;AN Guangxin;LI Zhilin;LIU Xiaotong;WANG Minghui;MENG Qingyi(School of Mechanical Engineering,Hebei University of Technology,Tianjin 300401,China;Department of New Energy,Tianjin Sino-German University of Applied Sciences,Tianjin 300350,China)

机构地区：[1]河北工业大学机械工程学院,天津300401 [2]天津中德应用技术大学新能源系,天津300350

出　　处：《分析试验室》2025年第4期524-532,共9页Chinese Journal of Analysis Laboratory

基　　金：河北省自然科学基金(B2021202038)资助。

摘　　要：单细胞分析可从细胞水平揭示疾病发生发展的机制,为临床提供新的治疗思路,细胞的分离与筛选则是该分析方法的前提。微流控技术具有小型化、低成本等优势,但用于细胞筛选时仍存在需要对细胞标记、影响活性、芯片结构复杂和限定细胞类型等问题。将光纤与微流控芯片集成,设计一种利用激光辐射力分选细胞的方法,可为单细胞分选带来新的机遇。利用COMSOL软件建立细胞筛选的仿真模型,耦合层流、电磁波以及流体粒子追踪3个物理场进行数值模拟,确定了实验参数及条件。在此基础上对单模光纤进行加工、处理后嵌入自主设计的微流控芯片,搭建了激光导向细胞筛选实验系统,成功分离了直径为7μm和15μm的2种聚苯乙烯小球以及酵母菌细胞。探究了不同直径的细胞在不同功率的激光作用下的偏移规律:保持其他条件不变,细胞偏移距离随着直径的增大而增加;对于同一直径细胞,细胞的偏移距离亦随激光功率的增大而增大,细胞的偏移距离和激光功率近似呈正比。本研究为基于微流控芯片和激光技术的细胞筛选提供了一种可行方案。Single-cell analysis can reveal the mechanisms of disease occurrence and development at cellular level,and therapeutic ideas for clinical practice.Cell separation and sorting are prerequisites for this analysis method.Microfluidic technology has the advantages of miniaturization and low cost,but it still has some problems when used for cell sorting,such as the need for cell labeling,affecting cell activity,complex chip structure and limited cell types.We integrate optical fiber and microfluidic chip and design a method of using laser radiation force to sort cells,which brings new opportunities for single-cell sorting.COMSOL software is used to establish a simulation model of cell sorting,coupling laminar flow,electromagnetic wave and particle to track three physical fields for performing numerical simulation,and the experimental parameters and conditions are determined.On this basis,singlemode optical fiber is embbed into a microfluidic chip,and a laser-guided cell sorting experimental system is built,which successfully separates two kinds of polystyrene spheres with diameters of 7μm and 15μm and yeast cells.The relationships between displacement and cells'diameter and laser power are explored,keeping other conditions unchanged,the cell displacement distance increasing with the increase of diameter,and for the same diameter cells,the displacement distance increasing linearly with the increase of laser power.This study provides a feasible scheme for cell non-destructive sorting based on microfluidic and laser technology.

关 键 词：激光导向 光阱力 细胞筛选 微流控系统 光纤嵌入 

分 类 号：TN249[电子电信—物理电子学] O439[机械工程—光学工程]