单次和双次富血小板血浆治疗卵巢储备功能减退的疗效  

Efficacy of single and double platelet-rich plasma treatment for diminished ovarian reserve

作　　者：彭婀娜 艾苗 谭小军 赵行平 徐大宝[3] PENG Enuo;AI Miao;TAN Xiaojun;ZHAO Xingping;XU Dabao(Reproductive Center,Third Xiangya Hospital,Central South University,Changsha 410013;Reproductive Center,Xiangtan Central Hospital,Xiangtan 411100;Department of Gynecology,Third Xiangya Hospital,Central South University,Changsha 410013,China)

机构地区：[1]中南大学湘雅三医院生殖中心,长沙410013 [2]湘潭市中心医院生殖中心,湘潭411100 [3]中南大学湘雅三医院妇科,长沙410013

出　　处：《中南大学学报(医学版)》2025年第1期45-51,共7页Journal of Central South University :Medical Science

基　　金：湖南省科技创新计划项目(2021SK52404)。

摘　　要：目的:研究报道富血小板血浆(platelet-rich plasma,PRP)治疗能改善女性卵巢功能,但其使用剂量和频次与治疗效果的关系尚不明确。本研究旨在评估单次和双次PRP治疗卵巢储备功能减退(diminished ovarian reserve,DOR)的疗效。方法:选取2020年9月至2022年10月湘潭市中心医院生殖中心收治的DOR患者65例,随机分为4组:单次PRP治疗组(21例,PRP治疗1次)、双次PRP治疗组(15例,PRP连续治疗2次)、空白对照组(15例)、人工周期对照组(14例,雌孕激素治疗)。比较4组患者治疗前后基础卵泡刺激素(follicle-stimulating hormone,FSH)水平、窦卵泡数(antral follicle count,AFC)、卵巢体积,行单精子卵细胞质内注射(intracytoplasmic sperm injection,ICSI)周期前后的获卵数、MII卵母细胞数、优质胚胎数及妊娠情况的差异。结果:与治疗前相比,单次和双次PRP治疗组的FSH水平均显著降低,AFC、卵巢体积、获卵数和MII卵母细胞数均显著增加(均P<0.05);与空白对照组相比,单次和双次PRP注射组治疗后的FSH水平降低,AFC、获卵数及MII卵母细胞数均增加(均P<0.05),单次和双次PRP注射组的优胚率、临床妊娠率和活产率均大于空白对照组及人工周期对照组,但差异均无统计学意义(均P>0.05);与单次PRP注射组相比,双次PRP注射组的FSH水平更低,AFC更多,但差异均无统计学意义(均P>0.05)。结论:单次和双次PRP卵巢注射均能有效改善DOR患者的卵巢储备功能,提高卵巢反应性;与单次PRP卵巢注射相比,双次PRP卵巢注射在改善卵巢储备功能方面有取得更好效果的趋势。Objective:Platelet-rich plasma(PRP)treatment has been reported to improve ovarian function in women,but the relationship between the dose and frequency of PRP treatment and its therapeutic effect remains unclear.This study aims to evaluate the efficacy of single and double PRP treatments for diminished ovarian reserve(DOR).Methods:A total of 65 patients treated at the Reproductive Center of Xiangtan Central Hospital from September 2020 to October 2022 were randomly divided into 4 groups:A single PRP treatment group(21 patients,PRP treatment once),a double PRP treatment group(15 patients,PRP treatment twice),a blank control group(15 patients),and an artificial cycle control group(14 patients,treated with estrogen and progesterone).The differences in baseline follicle-stimulating hormone(FSH),antral follicle count(AFC),ovarian volume,number of oocytes retrieved,number of good quality embryos,and pregnancy outcomes before and after treatment were compared among the 4 groups.Results:Compared to before treatment,both single and double PRP treatment groups showed a significant reduction in FSH,and an increase in AFC,ovarian volume,numbers of oocytes retrieved,and number of MII oocytes(all P<0.05).Compared to the blank control group,the single and double PRP groups showed a decrease in FSH,with an increase in AFC,number of oocytes retrieved,and number of MII oocytes(all P<0.05).The rates of good quality embryos,clinical pregnancy rate,and live birth rate in the single and double PRP groups were higher than those in the blank control group and artificial cycle control groups,but the differences were not statistically significant(all P>0.05).Compared to the single PRP treatment group,the double PRP group had lower FSH and higher AFC,but the differences were not statistically significant(both P>0.05).Conclusion:Both single and double PRP ovarian injections can effectively improve ovarian reserve function in DOR patients and enhance ovarian response.Compared to single PRP ovarian injection,double PRP ovarian injection shows

关 键 词：卵巢储备功能减退 富血小板血浆 卵巢储备 性激素 单精子卵细胞质内注射 

分 类 号：R711.6[医药卫生—妇产科学]