光谱法和色谱法对原花青素含量测试的比对研究  

Comparison study of spectroscopy and chromatography for proanthocyanidin content testing

作　　者：钟雪莲 何志明 ZHONG Xuelian;HE Zhiming(Guangzhou Inspecting Testing and Certification Group Co.,Ltd.,Guangzhou 511447,Guangdong,China)

机构地区：[1]广州检验检测认证集团有限公司,广东广州511447

出　　处：《中国纤检》2025年第4期55-57,共3页China Fiber Inspection

基　　金：广州市市场监督管理局科技创新项目“板蓝根等百草纤维产品中活性物质检测研究”(2023KJ53)。

摘　　要：本文通过线性相关性、灵敏度、精密度和抗干扰能力试验,比较了采用分光光度法和高效液相色谱法检测原花青素的优劣。结果表明:分光光度法的线性相关系数为0.9994,高效液相色谱法的线性相关系数为0.9998,连续重复进样分光光度法的RSD为2.9%,高效液相色谱法为1.9%,线性相关性、精密度两种仪器分析方法都能满足测试要求;灵敏度测试中高效液相色谱法优于分光光度法,高效液相色谱法检出限为2.0 mg/L,分光光度法检出限为5.0 mg/L;对于一些水解反应后存在颜色干扰的样品,高效液相色谱法定性结果更准确。In this paper,through experiments on linear correlation,sensitivity,precision and anti-interference ability,the advantages and disadvantages of spectrophotometry and high-performance liquid chromatography in the detection of proanthocyanidins were compared.The linear correlation coefficient of spectrophotometry is 0.994,and that of high-performance liquid chromatography is 0.998.The relative standard deviation(RSD)of continuous repeated injections by spectrophotometry is 2.9%,while that of high-performance liquid chromatography is 1.9%.Both instrumental analysis methods of linear correlation and precision can meet the testing requirements.In the sensitivity test,HPLC was superior to spectrophotometry.The detection limits of HPLC were 2.0 mg/L,and those of spectrophotometry were 5.0 mg/L.For some samples with color interference after hydrolysis reac-tions,the qualitative results obtained by high-performance liquid chromatography are more accurate.

关 键 词：原花青素 分光光度法 高效液相色谱法 抗干扰 准确性 

分 类 号：TS107[轻工技术与工程—纺织工程]