人血浆脂蛋白(a)的分离纯化  

Isolation and purification of human plasma lipoprotein(a)

作　　者：贺佳佳 苏燕[1] 丁海麦[1] 王雪妍 王安杰 韩润林[2] 刘治 HE Jiajia;SU Yan;DING Haimai;WANG Xueyan;WANG Anjie;HAN Runlin;LIU Zhi(Department of Biochemistry and Molecular Biology,Baotou Medical College,Baotou 014040,China;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010010,China)

机构地区：[1]包头医学院生物化学与分子生物学教研室,包头014040 [2]内蒙古农业大学兽医学院,呼和浩特010010

出　　处：《生命的化学》2025年第4期734-740,共7页Chemistry of Life

基　　金：内蒙古自治区自然科学基金项目(2022QN08018);内蒙古自治区高等学校科学研究项目(NJZZ22063)。

摘　　要：血浆脂蛋白(a)[lipoprotein(a),Lp(a)]的制备是其功能研究的重要基础。高效的分离纯化方法对于Lp(a)的实验室研究及临床检测至关重要。本文在以往研究的基础上,将不连续密度梯度超速离心技术与阴离子交换层析技术相结合,建立了一种实验室可行的、快速、简便、稳定的Lp(a)分离纯化方法。结果表明,该方法分离纯化得到的Lp(a)纯度较高、组分完整、提取效率稳定,可以为Lp(a)相关的科学研究提供可靠的实验材料。The preparation of plasma lipoprotein(a)[Lp(a)]is an important foundation for its functional research,and efficient separation and purification methods are crucial for laboratory studies and clinical testing of Lp(a).Based on previous research,this paper combined discontinuous density gradient ultracentrifugation with anion exchange chromatography to establish a fast,simple,and stable method for the separation and purification of Lp(a)that was feasible in the laboratory.The results showed that Lp(a)obtained by this method had high purity,complete components,and stable extraction efficiency,providing reliable experimental materials for Lp(a)-related scientific research.

关 键 词：脂蛋白(a) 密度梯度超速离心 阴离子交换层析 

分 类 号：R446.1[医药卫生—诊断学]