十字花科植物调控硫苷合成MYB转录因子的生物信息学分析  

Bioinformatics analysis of MYB transcription factors regulating glucosinolate biosynthesis in Brassicaceae

作　　者：李春金 何靖华 张欣瑶 曹睿 宋小明 Li Chunjin;He Jinghua;Zhang Xinyao;Cao Rui;Song Xiaoming(School of Life Sciences,North China University of Science and Technology,Tangshan,Hebei 063210,China)

机构地区：[1]华北理工大学生命科学学院,河北唐山063210

出　　处：《东南园艺》2025年第1期1-14,共14页Southeast Horticulture

基　　金：国家自然科学基金项目(32172583);中央引导地方科技发展资金项目(246Z2509G);河北省杰出青年基金项目(C2022209010);河北省省属高校基本科研业务费基金项目(JJC2024001)。

摘　　要：【目的】本研究聚焦于十字花科植物中调控硫苷(Glucosinolate,GSL)合成的MYB转录因子,旨在系统分析其进化动态、基因在染色体上的分布、共线性关系及基因表达模式,以揭示硫苷合成途径的进化机制及其在植物适应性中的作用。【方法】对29个染色体水平的十字花科物种构建物种树,分析全基因组加倍事件对MYB转录因子数量的影响。在29个物种中鉴定与硫苷合成相关的MYB转录因子,并对芸薹族中的相关转录因子进行系统发育和结构域分析。进行染色体定位分析,研究这些转录因子在染色体上的分布特征。开展共线性分析,揭示这些基因在不同物种间的进化保守性与分化特征。分析基因表达模式,研究它们在不同组织中的表达特异性。【结果】全基因组加倍事件显著增加了MYB转录因子的数量,尤其是在芸薹族中。在29个物种中共鉴定出322个与硫苷合成相关的MYB转录因子,其中芸薹族植物中鉴定到176个。芸薹族中与硫苷合成相关的MYB转录因子在进化树上呈现显著的聚类特征,表明其在进化过程中保留了相似的结构与功能。染色体定位分析显示,这些转录因子主要分布在染色体末端,可能与其表达调控的灵活性相关。共线性分析揭示了这些基因在不同物种间的进化保守性与分化特征。基因表达模式分析发现它们在不同组织中表现出显著的特异性表达。【结论】本研究为理解芸薹族植物硫苷合成途径的进化机制及其在植物适应性中的作用提供了重要的分子基础,并为后续功能验证和应用研究提供了新视角。【Objective】This study focuses on MYB transcription factors(TFs)that regulate the biosynthesis of glucosinolates(GSL)in Brassicaceae,aiming to systematically analyze their evolutionary dynamics,chromosomal distribution,collinearity,and gene expression patterns to reveal the evolutionary mechanisms of the glucosinolate biosynthetic pathway and its role in plant adaptation.【Method】A species tree was constructed for 29 chromosome-level species to analyze the impact of whole-genome duplication events on the number of MYB TFs.MYB TFs related to glucosinolate biosynthesis were identified in 29 species,and a phylogenetic and domain structure analysis was performed on the relevant transcription factors in the tribe Brassiceae.Chromosomal localization analysis was conducted to study the distribution characteristics of these transcription factors on chromosomes.Collinearity analysis was performed to reveal the evolutionary conservation and differentiation of these genes among different species.Gene expression patterns were analyzed to study their tissue-specific expression.【Result】Whole-genome duplication events significantly increased the number of MYB TFs,especially in the tribe Brassiceae.A total of 322 MYB TFs related to glucosinolate biosynthesis were identified in 29 species,among which 176 were identified in Brassiceae tribe.The MYB TFs related to glucosinolate biosynthesis in Brassiceae tribe showed significant clustering on the phylogenetic tree,indicating that they retained similar structures and functions during evolution.Chromosomal localization analysis revealed that these transcription factors were mainly distributed at the ends of chromosomes,which may be related to the flexibility of their expression regulation.Collinearity analysis revealed the evolutionary conservation and differentiation of these genes among different species.Gene expression pattern analysis showed that they exhibited significant tissue specific expression.【Conclusion】This study provides an important molecular basis for un

关 键 词：十字花科 芸薹族 硫苷 MYB转录因子 进化机制 合成途径 染色体 表达调控 

分 类 号：S63[农业科学—蔬菜学]