自然储存条件下水稻种子耐储藏性状QTL的定位  

QTL mapping for seed storage tolerance trait in rice under natural conditions

作　　者：陈文凤 黄浩 丁晓雯 林晋军 姚威 陈倩茹 黄希来 刘雄伦[1,2] 吴俊[1,2] 陈哲红 程计华 刘金灵 CHEN Wenfeng;HUANG Hao;DING Xiaowen;LIN Jinjun;YAO Wei;CHEN Qianru;HUANG Xilai;LIU Xionglun;WU Jun;CHEN Zhehong;CHENG Jihua;LIU Jinling(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Hunan Provincial Key Laboratory of Crop Gene Engineering,Changsha,Hunan 410128,China;Huazhi Biotechnology Co.Ltd.,Changsha,Hunan 410125,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]作物基因工程湖南省重点实验室,湖南长沙410128 [3]华智生物技术有限公司,湖南长沙410125

出　　处：《湖南农业大学学报(自然科学版)》2025年第2期1-5,35,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31972256);湖南省2023十大技术攻关项目(2023NK1010);苏州市科技计划项目(SNG2021003)。

摘　　要：为了筛选出新的水稻耐储藏种质,以在自然条件下储存4年的由粳稻‘魔王谷’(MWG)和籼稻‘CO39’杂交后代衍生的重组自交系群体种子为材料,对群体种子发芽表型进行分析,并利用复合区间作图法对该群体种子耐储藏性状QTL进行定位。结果表明:101份重组自交系中,67%的株系种子的发芽率、发芽势和发芽指数为0,其余株系3个发芽性状指标分别为0~82.67%、0~46.00%和0~6.48;鉴定到1个发芽率达82.67%的高耐储株系;QTL分析定位到3个控制发芽性状(发芽率、发芽势、发芽指数)的QTL,分别为qGR7、q GP7和qGI7,其中qGR7位于SSR标记RM6344与RM5720区间18.43c M的区域,qGP7和qGI7均位于标记RM5672与RM8261区间35.74 cM的区域,3个QTL的LOD值为2.52~2.56,表型贡献率为12.02%~12.22%。To screen new rice storage tolerance germplasm,seed of one recombinant inbred lines(RILs)population that had been in natural storage for 4 years and derived from the cross between japonica cultivar‘Mowanggu’(MGW)and indica rice‘CO39’was used to analyze the germination phenotype of seed from the population and to map QTLs responsible for seed storage tolerance trait by composite interval mapping.The results showed that,the phenotype of germination rate,germination potential,and germination index of 67%lines from the 101 RIL lines was declined to 0.The value of the 3 traits for other lines ranged from 0-82.67%,0-46.00%and 0-6.48 respectively.Only one line still keeps a high germination rate of 82.67%.QTL mapping yielded 3 QTLs responsible for germination traits,namely qGP7,qGE7 and qGI7.Among them,qGR7 was mapped in the interval of SSR marker RM6344 and RM5720 with a distance of 18.43 cM,qGP7 and qGI7 located in the same interval between SSR maker RM5672 and RM8261 with a distance of 35.74 cM.The LOD values of 3 QTLs ranged from 2.52-2.56,of which the phenotypic contribution rate was 12.02%-12.22%.

关 键 词：水稻 耐储藏性 重组自交系群体 数量性状基因位点 基因定位 

分 类 号：S511.01[农业科学—作物学]