早期宫颈癌组织HOXD3、Septin-9蛋白表达变化及其与淋巴结转移的关系  

Expression changes of homeobox gene D3 and Septin-9 proteins in early cervical cancer tissues and their relationship with lymph node metastasis

作　　者：向黎 刘桥梅 马灵 吴忠均 朱玉霖 XIANG Li;LIU Qiaomei;MA Ling;WU Zhongjun;ZHU Yulin(Department of Pathology,Yibin Sixth People′s Hospital,Yibin 644600,Sichuan,China;Department of Pathology,Jianwei County People′s Hospital,Leshan 614400,Sichuan,China)

机构地区：[1]宜宾市第六人民医院病理科,四川宜宾644600 [2]犍为县人民医院病理科,四川乐山614400

出　　处：《中国性科学》2025年第4期66-70,共5页Chinese Journal of Human Sexuality

基　　金：2021年四川省医学(青年创新)科研课题项目(S21109)。

摘　　要：目的探讨早期宫颈癌组织同源盒基因D3(HOXD3)、Septin-9蛋白表达变化及其与淋巴结转移的关系。方法选取2020年3月至2024年3月宜宾市第六人民医院收治的70例已开展广泛性子宫切除术的早期宫颈癌患者作为研究对象,根据术后证实是否有淋巴结转移分为转移组(n=24)和未转移组(n=46)。获取两组宫颈癌标本及癌旁正常组织后检测HOXD3、Septin-9蛋白阳性表达及定量表达水平。比较宫颈癌组织及癌旁正常组织HOXD3、Septin-9蛋白阳性表达率,比较两组临床特征及HOXD3信使RNA(mRNA)、Septin-9 mRNA相对表达情况,采用受试者工作特征(ROC)曲线分析HOXD3 mRNA、Septin-9 mRNA评估患者淋巴结转移的诊断价值,采用Logistic回归分析早期宫颈癌患者淋巴结转移的危险因素。结果早期宫颈癌组织HOXD3、Septin-9蛋白阳性表达率均高于癌旁正常组织(P<0.05)。转移组肿瘤直径>3 cm、深间质浸润比例、HOXD3 mRNA、Septin-9 mRNA相对表达水平均高于未转移组(P<0.05)。HOXD3 mRNA评估患者淋巴结转移预测性能较高(曲线下面积=0.912,P<0.05),Septin-9 mRNA具有一定预测性能(曲线下面积=0.894,P<0.05)。肿瘤直径>3 cm、深间质浸润、HOXD3 mRNA>0.145、Septin-9 mRNA>1.975是早期宫颈癌患者出现淋巴结转移的危险因素(P<0.05)。结论HOXD3、Septin-9蛋白在早期宫颈癌组织中阳性表达率较高,且较大肿瘤直径、深间质浸润、高HOXD3 mRNA、高Septin-9 mRNA表达水平是早期宫颈癌患者出现淋巴结转移的危险因素。Objective To explore expression changes of homeobox gene D3(HOXD3)and Septin-9 proteins in early cervical cancer tissues and their relationship with lymph node metastasis.Methods A total of 70 patients with early cervical cancer undergoing extensive hysterectomy in Yibin Sixth People′s Hospital between March 2020 and March 2024 were enrolled as the research objects.According to presence or absence of postoperative lymph node metastasis,they were divided into metastasis group(n=24)and non-metastasis group(n=46).The specimens of cervical cancer and normal para-carcinoma tissues were collected to detect positive and quantitative expression levels of HOXD3 and Septin-9 proteins.The positive expression rates of HOXD3 and Septin-9 proteins in cervical cancer tissues and normal para-carcinoma tissues were compared.The clinical characteristics and relative expression condition of HOXD3 messenger RNA(mRNA)and Septin-9 mRNA in the two groups were compared.The evaluation value of HOXD3 mRNA and Septin-9 mRNA in lymph node metastasis was analyzed by receiver operating characteristic(ROC)curves,and risk factors of lymph node metastasis were analyzed by Logistic regression analysis.Results The positive expression rates of HOXD3 and Septin-9 proteins in early cervical cancer tissues were higher than those in normal para-carcinoma tissues(P<0.05).The proportions of tumor diameter>3 cm and deep interstitial infiltration,and relative expression levels of HOXD3 mRNA and Septin-9 mRNA in metastasis group were higher than those in non-metastasis group(P<0.05).The predictive efficiency of HOXD3 mRNA was high for evaluating lymph node metastasis in patients[area under the curve(AUC)=0.912,P<0.05],while Septin-9 mRNA had certain predictive efficiency(AUC=0.894,P<0.05).Tumor diameter>3 cm,deep interstitial infiltration,HOXD3 mRNA>0.145 and Septin-9 mRNA>1.975 were risk factors of lymph node metastasis in patients with early cervical cancer.Conclusions The positive expression rates of HOXD3 and Septin-9 proteins are higher in early cerv

关 键 词：同源盒基因D3 Septin-9 宫颈癌 淋巴结转移 

分 类 号：R711[医药卫生—妇产科学]