3D细胞培养系统在顶复门原虫研究中的应用  

Application of the 3D Cell Culture System in the Study of Apicomplexa Protozoa

作　　者：谢月华 谢福杰 索勋[2] 闫文朝[1] XIE Yuehua;XIE Fujie;SUO Xun;YAN Wenchao(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China;College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)

机构地区：[1]河南科技大学动物科技学院,洛阳471003 [2]中国农业大学动物医学院,北京100193

出　　处：《畜牧兽医学报》2025年第4期1540-1548,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31772733)。

摘　　要：顶复门原虫包含隐孢子虫、弓形虫、疟原虫、艾美耳球虫等和人类、动物健康相关的寄生虫。它们可以感染广泛的宿主且不易被免疫系统发现,引起急性和慢性疾病。由于其在自然宿主中进行研究的伦理和实际问题,加之顶复门原虫具有复杂的生活史且动物模型建立通常比较困难,使得人们对这些病原体生物学特性的了解仍然有限。最近使用的3D细胞培养系统促进和拓展了顶复门原虫的研究,使人们能模拟宿主-病原体互作,为疫苗开发、药物筛选、寄生虫生物学研究及其他应用提供平台。本综述总结了3D细胞培养系统应用在顶复门原虫研究中的进展,以期为读者从事相关研究提供参考。The protozoa of Apicomplexa include Cryptosporidium,Toxoplasma,Plasmodium,Eimeria and other parasites related to human and animal health.They can infect a wide range of hosts and are not easy to be found by the immune system,causing acute and chronic diseases.Due to the ethical and practical problems of its research in natural reservoir,as well as the complex life circle of apicomplexan protozoa and the difficulty of animal models,people's understanding of the biological characteristics of these pathogens is still limited.The recently used 3D system has promoted and expanded the research of apicomplexan protozoa,enabling us to simulate host pathogen interaction,providing a platform for vaccine development,drug-screening,parasitic biology research and other applications.This review summarizes the progress in the application of 3D cell culture system in the study of apicomplexan protozoa,with a view to providing reference for subsequent related researches.

关 键 词：3D细胞培养系统 隐孢子虫 弓形虫 疟原虫 艾美耳球虫 

分 类 号：Q813.11[生物学—生物工程] S852.723[农业科学—基础兽医学]