环介导等温扩增技术的发展及其在耐药基因检测中的应用  

Development of Ring-mediated Isothermal Amplification Technology and Its Application to the Detection of Drug Resistance Genes

作　　者：廖怡雯 叶景芬 武绍碧 陈世雄 杨婉 罗雪 杨琦[1] LIAO Yiwen;YE Jingfen;WU Shaobi;CHEN Shixiong;YANG Wan;LUO Xue;YANG Qi(College of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学动物科学学院,贵阳550025

出　　处：《畜牧兽医学报》2025年第4期1621-1631,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(32260884)。

摘　　要：本文介绍了环介导等温扩增(loop-mediated isothermal amplification, LAMP)技术的原理、过程和特点,以及其在耐药基因检测中的应用。LAMP技术以其高效、特异、灵敏和简便的特点,在分子生物学领域得到了广泛应用。除了基本的LAMP检测方法外,本文还对一些新型LAMP技术,如IMS-LAMP、LAMP与CRISPR/Cas系统的联用、LAMP与Argonaute系统的联用、LAMP传感器以及LAMP与微流控芯片的结合等做了介绍。针对耐药基因的检测中,LAMP技术展现了很好的应用前景,为快速、准确地检测耐药基因提供了有效的工具。This paper describes the principle,process and characteristics of loop-mediated isothermal amplification(LAMP)technology and its application in the detection of drug resistance genes.LAMP technology has been widely used in the field of molecular biology due to its high efficiency,specificity,sensitivity and simplicity.In addition to the basic LAMP detection methods,some novel LAMP technologies,such as IMS-LAMP,LAMP coupled with CRISPR/Cas system,LAMP coupled with Argonaute system,LAMP sensors,and LAMP coupled with microfluidic chip,are also introduced in this paper.In addition,for the detection of drug-resistant genes,LAMP technology shows a good application prospect in the detection of drug resistance genes,which provides an effective tool for rapid and accurate detection of drug resistance genes.

关 键 词：环介导等温扩增 恒温扩增 快速扩增 检测方法 耐药基因检测 

分 类 号：S852.6[农业科学—基础兽医学]